ARG66739
anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody [CR3022]
anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody [CR3022] for ELISA,ICC/IF,Neutralizing and Virus
Overview
| Product Description | Recombinant Human Monoclonal antibody [CR3022] recognizes SARS-CoV / SARS-CoV-2 Spike protein (RBD) |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | ELISA, ICC/IF, Neut |
| Specificity | This antibody binds the amino acids 318-510 in the S1 domain (RBD, Receptor Binding Domain) of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. The antibody also binds to P462L-substituted S318–510 fragments of the SARS spike protein. The binding epitope is only accessible in the "open" conformation of the spike protein (Joyce et al. 2020) |
| Host | Human |
| Clonality | Monoclonal |
| Clone | CR3022 |
| Isotype | IgG1 |
| Target Name | SARS-CoV / SARS-CoV-2 Spike protein (RBD) |
| Antigen Species | Virus |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | Neutralizing: Clone CR3022 is the first anti-SARS-CoV neutralizing antibody to cross-react with SARS-CoV-2. Structural modeling has confirmed that CR3022 targets a conserved epitope between SARS-CoV and SARS-CoV2 in the RBD domain (PMID: 32245784, 32065055). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from antibody expressing HEK293 cell culture medium. |
| Purity | > 98% (SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Images (2) Click the Picture to Zoom In
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ARG66739 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody [CR3022] ICC/IF image
Immunofluorescence: 293T cells were transfected with Mock or SARS-CoV-2 Spike expression plasmid. Cells were stained with ARG66739 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody [CR3022] (green). DAPI (blue) for nuclear staining.
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ARG66739 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody [CR3022] ELISA image
ELISA: Titration of ARG66739 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody [CR3022].
RBD = Receptor binding domain
