ARG43343
anti-SARS-CoV-2 Spike protein (S2) antibody
anti-SARS-CoV-2 Spike protein (S2) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Virus
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SARS-CoV-2 Spike protein (S2) |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SARS-CoV-2 Spike protein (S2) |
| Antigen Species | Virus |
| Immunogen | A 20-amino acid synthetic peptide within the last 50 amino acids of SARS-CoV-2 Spike protein (S2). |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (3) Click the Picture to Zoom In
-
ARG43343 anti-SARS-CoV-2 Spike protein (S2) antibody ICC/IF image
Immunofluorescence: 4% Paraformaldehyde-fixed 293T cells stained with ARG43343 anti-SARS-CoV-2 Spike protein (S2) antibody (green) at 1 µg/ml dilution. DAPI (blue) for nuclear staining.
-
ARG43343 anti-SARS-CoV-2 Spike protein (S2) antibody IHC-P image
Immunohistochemistry: Formaldehyde-fixed and paraffin-embedded COVID-19 patient lung tissue. Tissue was blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Samples were stained with ARG43343 anti-SARS-CoV-2 Spike protein (S2) antibody at 0.5 µg/ml diluiton, overnight at 4°C. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages and airway epithelium of COVID-19 patient lung, but not in non-COVID-19 patient lung.
-
ARG43343 anti-SARS-CoV-2 Spike protein (S2) antibody WB image
Western blot: Wild type 293 cells (left) and SARS-CoV-2 Spike overexpressed 293 cells (right). 15 µg of cell lysates stained with ARG43343 anti-SARS-CoV-2 Spike protein (S2) antibody at 1 µg/ml dilution, 1 hour incubation at RT in 5% NFDM/TBST.
