ARG43345
anti-SARS-CoV-2 Spike protein (RBD) antibody
anti-SARS-CoV-2 Spike protein (RBD) antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Virus
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SARS-CoV-2 Spike protein (RBD) |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | ELISA, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SARS-CoV-2 Spike protein (RBD) |
| Antigen Species | Virus |
| Immunogen | A 19-amino acid synthetic peptide within the last 50 amino acids of SARS-CoV-2 Spike protein (RBD). |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (4) Click the Picture to Zoom In
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ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody ICC/IF image
Immunofluorescence: 4% Paraformaldehyde-fixed Spike transfected 293 cells stained with ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody (green) at 20 µg/ml dilution. DAPI (blue) for nuclear staining.
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ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody IHC-P image
Immunohistochemistry: Formaldehyde-fixed and paraffin-embedded COVID-19 patient lung tissue. Tissue was blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Samples were stained with ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody at 0.5 µg/ml dilution, overnight at 4°C. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Spike RBD protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
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ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody WB image
Western blot: 30 ng of SARS-CoV-2 Spike RBD recombinant protein stained with ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody at 1 µg/ml (left) or 2 µg/ml (right) dilution, for 1 hour incubation at RT, in 5% NFDM/TBST.
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ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody ELISA image
Direct ELISA: SARS-CoV-2 Spike RBD recombinant protein as coating antigen and ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody as the primary antibody.
