ARG43303
anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody
anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Virus
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SARS-CoV-2 NSP5 / 3CLpro protein |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | ELISA, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SARS-CoV-2 NSP5 / 3CLpro protein |
| Antigen Species | Virus |
| Immunogen | A 16-amino acid synthetic peptide within the first 50 amino acids of SARS-CoV-2 NSP5 / 3CLpro protein. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (3) Click the Picture to Zoom In
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ARG43303 anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody IHC-P image
Immunohistochemistry: Paraffin-embedded COVID-19 patient lung tissue. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Samples were stained with ARG43303 anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody at 0.5 µg/ml dilution, overnight at 4°C. Counter stained with Hematoxylin.
Strong signal of SARS-COV-2 NSP5 / 3Lpro protein was observed in macrophages of COVID-19 patient lung (left), but not in non-COVID-19 patient lung (right).
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ARG43303 anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody WB image
Western blot: 30 ng of SARS-CoV-2 NSP5 / 3CLpro recombinant protein stained with ARG43303 anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody, for 1 hour at RT in 5% NFDM/TBST. Antibody dilutions: 0.5 µg/ml (left) and 1 µg/ml (right).
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ARG43303 anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody ELISA image
Direct ELISA: SARS-CoV-2 NSP5 / 3CLpro recombinant protein as coating antigen and serial diluted ARG43303 anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody as capture antibody.
