ARG43205
anti-SARS-CoV-2 Envelope protein antibody
anti-SARS-CoV-2 Envelope protein antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Virus
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SARS-CoV-2 Envelope protein |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | ELISA, IHC-P, WB |
| Specificity | This antibody can detect 2 ng of free peptide at 1 µg/ml in ELISA. It can detect SARS-CoV-2 Envelope recombinant protein by ELISA and WB. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SARS-CoV-2 Envelope protein |
| Antigen Species | Virus |
| Immunogen | A 14-amino acid peptide within the last 50 amino acids of SARS-CoV-2 Envelope protein. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | Direct ELISA: Detection range: 1 - 3000 ng/ml (recombinant protein); 0.3 - 1000 ng/ml (immunogen peptide). IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Highlight | Related products: SARS-CoV antibodies; SARS-CoV ELISA Kits; SARS-CoV recombinant proteins; Anti-Rabbit IgG secondary antibodies; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 |
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Images (4) Click the Picture to Zoom In
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ARG43205 anti-SARS-CoV-2 Envelope protein antibody IHC-P image
Immunohistochemistry: Paraffin-embedded COVID-19 patient lung tissue. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG43205 anti-SARS-CoV-2 Envelope protein antibody at 0.5 ug/ml dilution, overnight at 4°C. Counter stained with Hematoxylin.
Strong signal of SARS-COV-2 envelope protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
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ARG43205 anti-SARS-CoV-2 Envelope protein antibody WB image
Western blot: 50 ng of SARS-CoV-2 Envelope recombinant protein (N-BBP-6xHis tagged) stained with ARG43205 anti-SARS-CoV-2 Envelope protein antibody for 1 hour incubation at RT in 5% NFDM/TBST, at 1 µg/ml (left) or 2 µg/ml (right) dilution.
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ARG43205 anti-SARS-CoV-2 Envelope protein antibody ELISA image
Direct ELISA: SARS-CoV-2 Envelope recombinant protein as coating antigen. Samples were stained with ARG43205 anti-SARS-CoV-2 Envelope protein antibody. Detection range: 1 - 3000 ng/ml.
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ARG43205 anti-SARS-CoV-2 Envelope protein antibody ELISA image
Direct ELISA: An immunogen peptide as coating antigen. Samples were stained with ARG43205 anti-SARS-CoV-2 Envelope protein antibody. Detection range: 0.3 - 1000 ng/ml.
