ARG52420
anti-S6 Kinase 1 phospho (Thr449) antibody
anti-S6 Kinase 1 phospho (Thr449) antibody for Western blot and Arabidopsis
Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes S6 Kinase 1 phospho (Thr449) |
|---|---|
| Tested Reactivity | Arabi |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | S6 Kinase 1 |
| Antigen Species | Arabidopsis |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr449 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 1; ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 6; ATPK1; ATPK6; ATS6K1; P70 RIBOSOMAL S6 KINASE; PK1; PK6; PROTEIN-SERINE KINASE; protein-serine kinase 1; ROTEIN-SERINE KINASE 6; S6K1 |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~53k S6K1 protein phosphorylated at Thr 449. The immunolabeling of the S6K1 band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | S6K1 |
| Background | Ribosomal s6 kinase is a member of a family of protein kinases involved in signal transduction. The subfamily S6K has two known homologues: S6K1 and S6K2. First characterized in mammals, S6K1 is controlled by target-of-rapamycin (TOR) kinase, which plays a central regulatory role in growth signaling pathways (Dufner and Thomas 1999). Osmotic stress inhibition of S6K is mediated by the TOR kinase pathway (Mahfouz et al., 2006). The activation of mammalian S6K1 involves phosphorylation at thr389 (Pearson et al., 2005), however its orthologue in Arabidopsis suggests that plant S6K1 thr449 is its functional equivalent (Schepetilnikov et al., 2011). The phytohormone auxin triggers TOR activation, which is followed by S6K1 phosphorylation at thr449, which in turn is critical for translation reinitiation (Schepetilnikov et al., 2013). Rapamycin effectively inactivates S6K1 thr449 phosphorylation in Arabidopsis seedlings, which suppresses TOR PK activity and ultimately plant growth (Xiong Y and Sheen J, 2011). |
| Research Area | Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 53 kDa |
Images (1) Click the Picture to Zoom In
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ARG52420 anti-S6 Kinase 1 phospho (Thr449) antibody WB image
Western blot: Arabidopsis lysate showing specific immunolabeling of the ~53k S6K1 phosphorylated at Thr449 by using ARG52420 anti-S6 Kinase 1 phospho (Thr449) antibody.
Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (P-peptide), but not by the corresponding nonphospho-peptide (NP-peptide).
