ARG41965

anti-S100A4 antibody [S100A4/1482]

anti-S100A4 antibody [S100A4/1482] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Overview

Product Description Mouse Monoclonal antibody [S100A4/1482] recognizes S100A4
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone S100A4/1482
Isotype IgG1, kappa
Target Name S100A4
Antigen Species Human
Immunogen Recombinant protein of Human S100A4.
Conjugation Un-conjugated
Alternate Names S100 calcium-binding protein A4; Placental calcium-binding protein; 42A; MTS1; FSP1; CAPL; Metastasin; Protein S100-A4; P9KA; 18A2; Protein Mts1; Calvasculin; PEL98

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/10^6 cells
ICC/IF2 - 5 µg/ml
IHC-P2 - 5 µg/ml
WB1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size ~ 12 kDa

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20198 Mouse S100A4

GeneID: 6275 Human S100A4

Swiss-port # P07091 Mouse Protein S100-A4

Swiss-port # P26447 Human Protein S100-A4

Gene Symbol S100A4
Gene Full Name S100 calcium binding protein A4
Background The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Highlight Related products:
S100A4 antibodies; S100A4 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
New antibody panels for Myofibroblasts and CAFs
Calculated MW 12 kDa

Images (6) Click the Picture to Zoom In

  • ARG41965 anti-S100A4 antibody [S100A4/1482] ICC/IF image

    Immunofluorescence: Fixed and permeabilized HeLa cells stained with ARG41965 anti-S100A4 antibody [S100A4/1482] (green). Reddot (red) for nuclear staining.

  • ARG41965 anti-S100A4 antibody [S100A4/1482] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling. The tissue section was stained with ARG41965 anti-S100A4 antibody [S100A4/1482].

  • ARG41965 anti-S100A4 antibody [S100A4/1482] WB image

    Western blot: HeLa, A549 and A431 cell lysates stained with ARG41965 anti-S100A4 antibody [S100A4/1482].

  • ARG41965 anti-S100A4 antibody [S100A4/1482] WB image

    Western blot: HeLa cell lysate stained with ARG41965 anti-S100A4 antibody [S100A4/1482].

  • ARG41965 anti-S100A4 antibody [S100A4/1482] FACS image

    Flow Cytometry: Human T98G cells stained with ARG41965 anti-S100A4 antibody [S100A4/1482] (blue) or isotype control (red).

  • ARG41965 anti-S100A4 antibody [S100A4/1482] FACS image

    Flow Cytometry: Human A549 cells stained with ARG41965 anti-S100A4 antibody [S100A4/1482] (blue) or isotype control (red).