ARG41964

anti-S100A4 antibody

anti-S100A4 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes S100A4
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name S100A4
Antigen Species Human
Immunogen Synthetic peptide of Human S100A4.
Conjugation Un-conjugated
Alternate Names S100 calcium-binding protein A4; Placental calcium-binding protein; 42A; MTS1; FSP1; CAPL; Metastasin; Protein S100-A4; P9KA; 18A2; Protein Mts1; Calvasculin; PEL98

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100
ICC/IF1:50 - 1:200
IHC-P1:100 - 1:500
IP1:40
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 12 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20198 Mouse S100A4

GeneID: 24615 Rat S100A4

GeneID: 6275 Human S100A4

Gene Symbol S100A4
Gene Full Name S100 calcium binding protein A4
Background The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
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Calculated MW 12 kDa

Images (3) Click the Picture to Zoom In

  • ARG41964 anti-S100A4 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG41964 anti-S100A4 antibody.

  • ARG41964 anti-S100A4 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon carcinoma tissue stained with ARG41964 anti-S100A4 antibody.

  • ARG41964 anti-S100A4 antibody WB image

    Western blot: A375 cell lysate stained with ARG41964 anti-S100A4 antibody.