ARG66832

anti-Ret phospho (Tyr1062) antibody

anti-Ret phospho (Tyr1062) antibody for ELISA,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes Ret phospho (Tyr1062)
Tested Reactivity Hu
Predict Reactivity Ms, Rat
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Ret
Antigen Species Human
Immunogen Phosphospecific peptide around Tyr1062 (between aa. 1041-1090) of Human Ret.
Conjugation Un-conjugated
Alternate Names RET51; CDHF12; HSCR1; Proto-oncogene c-Ret; PTC; Proto-oncogene tyrosine-protein kinase receptor Ret; RET-ELE1; CDHR16; MEN2B; MEN2A; MTC1; EC 2.7.10.1; Cadherin family member 12

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:10000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.5% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5979 Human RET

Swiss-port # P07949 Human Proto-oncogene tyrosine-protein kinase receptor Ret

Gene Symbol RET
Gene Full Name ret proto-oncogene
Background This gene encodes a transmembrane receptor and member of the tyrosine protein kinase family of proteins. Binding of ligands such as GDNF (glial cell-line derived neurotrophic factor) and other related proteins to the encoded receptor stimulates receptor dimerization and activation of downstream signaling pathways that play a role in cell differentiation, growth, migration and survival. The encoded receptor is important in development of the nervous system, and the development of organs and tissues derived from the neural crest. This proto-oncogene can undergo oncogenic activation through both cytogenetic rearrangement and activating point mutations. Mutations in this gene are associated with Hirschsprung disease and central hypoventilation syndrome and have been identified in patients with renal agenesis. [provided by RefSeq, Sep 2017]
Function Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant integrins-regulated cell migration. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which induces inhibition of food-intake. Activates MAPK- and AKT-signaling pathways (PubMed:28846097, PubMed:28953886, PubMed:28846099). Isoform 1 in complex with GFRAL induces higher activation of MAPK-signaling pathway than isoform 2 in complex with GFRAL (PubMed:28846099). [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 124 kDa
PTM Autophosphorylated on C-terminal tyrosine residues upon ligand stimulation. Dephosphorylated by PTPRJ on Tyr-905, Tyr-1015 and Tyr-1062.

Proteolytically cleaved by caspase-3. The soluble RET kinase fragment is able to induce cell death. The extracellular cell-membrane anchored RET cadherin fragment accelerates cell adhesion in sympathetic neurons. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG66832 anti-Ret phospho (Tyr1062) antibody WB image

    Western blot: HepG2 cells untreated (left) or treated (right) with forskolin (40 nM for 30 min, overnight at 4°C). Cell lysates were stained with ARG66832 anti-Ret phospho (Tyr1062) antibody.

  • ARG66832 anti-Ret phospho (Tyr1062) antibody WB image

    Western blot: K562 cell lysates stained with ARG66832 anti-Ret phospho (Tyr1062) antibody. The lane on the right is blocked with the phospho peptide.