Overview

Product Description	Rabbit Polyclonal antibody recognizes Ran
Tested Reactivity	Hu, Ms, Rat
Tested Application	FACS, ICC/IF, IP, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	Ran
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human Ran.
Conjugation	Un-conjugated
Alternate Names	GTP-binding nuclear protein Ran; Androgen receptor-associated protein 24; TC4; ARA24; Ras-like protein TC4; Gsp1; Ras-related nuclear protein; GTPase Ran

Application Instructions

Application Suggestion	Tested Application Dilution FACS 1:100 ICC/IF 1:50 - 1:200 IP 1:50 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 19384 Mouse RAN GeneID: 5901 Human RAN GeneID: 84509 Rat RAN Swiss-port # P62826 Human GTP-binding nuclear protein Ran Swiss-port # P62827 Mouse GTP-binding nuclear protein Ran Swiss-port # P62828 Rat GTP-binding nuclear protein Ran More
Gene Symbol	RAN
Gene Full Name	RAN, member RAS oncogene family
Background	RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq, Jul 2008]
Function	GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases. [UniProt]
Cellular Localization	Nucleus. Nucleus envelope. Cytoplasm, cytosol. Cytoplasm. Melanosome. Note=Predominantly nuclear during interphase (PubMed:8421051, PubMed:12194828, PubMed:10679025). Becomes dispersed throughout the cytoplasm during mitosis (PubMed:8421051, PubMed:12194828). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). [UniProt]
Calculated MW	24 kDa

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