ARG41119

anti-RPL21 antibody

anti-RPL21 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes RPL21
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RPL21
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-160 of Human RPL21 (NP_000973.2).
Conjugation Un-conjugated
Alternate Names L21; 60S ribosomal protein L21; HYPT12

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Raji
Observed Size 18 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6144 Human RPL21

GeneID: 79449 Rat RPL21

Swiss-port # P20280 Rat 60S ribosomal protein L21

Swiss-port # P46778 Human 60S ribosomal protein L21

Gene Symbol RPL21
Gene Full Name ribosomal protein L21
Background Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L21E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Cellular Localization Cytoplasm, cytosol. Cytoplasm. Endoplasmic reticulum. Note=Detected on cytosolic polysomes (PubMed:25957688). Detected in ribosomes that are associated with the rough endoplasmic reticulum (By similarity). [UniProt]
Calculated MW 19 kDa

Images (3) Click the Picture to Zoom In

  • ARG41119 anti-RPL21 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG41119 anti-RPL21 antibody at 1:200 dilution.

  • ARG41119 anti-RPL21 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG41119 anti-RPL21 antibody at 1:100 dilution.

  • ARG41119 anti-RPL21 antibody WB image

    Western blot: 25 µg of Raji cell lysate stained with ARG41119 anti-RPL21 antibody at 1:1000 dilution.