ARG44390

anti-RPL18 antibody

anti-RPL18 antibody for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Western blot,Flow cytometry and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes RPL18
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name RPL18
Antigen Species Human
Immunogen Human RPL18 recombinant protein (aa. sequence: K19-A177).
Conjugation Un-conjugated
Alternate Names RPL18; Ribosomal Protein L18; 60S Ribosomal Protein L18; L18; Large Ribosomal Subunit Protein EL18; EL18; DBA18

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/1x10^6 cells
ICC/IF5 µg/ml
IHC-P2-5 µg/ml
WB0.25-0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 19899 Mouse RPL18

GeneID: 6141 Human RPL18

GeneID: 81766 Rat RPL18

Gene Symbol RPL18
Gene Full Name Ribosomal Protein L18
Background Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18E family of ribosomal proteins that is a component of the 60S subunit. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Function Component of the large ribosomal subunit.
Cellular Localization Cytoplasm, Endoplasmic reticulum
Calculated MW 22 kDa
PTM Isopeptide bond, Phosphoprotein, Ubl conjugation

Images (8) Click the Picture to Zoom In

  • ARG44390 anti-RPL18 antibody IHC-P image

    Immunohistochemistry: Human rectum adenocarcinoma stained with ARG44390 anti-RPL18 antibody at 2 μg/ml dilution.

  • ARG44390 anti-RPL18 antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG44390 anti-RPL18 antibody at 5 μg/mL dilution.

  • ARG44390 anti-RPL18 antibody WB image

    Western blot: HeLa, 293T and HepG2 stained with ARG44390 anti-RPL18 antibody at 0.5 μg/mL dilution.

  • ARG44390 anti-RPL18 antibody FACS image

    Flow Cytometry: HeLa stained with ARG44390 anti-RPL18 antibody at 1 μg/10^6 cells dilution.

  • ARG44390 anti-RPL18 antibody IHC-P image

    Immunohistochemistry: Rat colon stained with ARG44390 anti-RPL18 antibody at 2 μg/ml dilution.

  • ARG44390 anti-RPL18 antibody WB image

    Western blot: Rat testis and Rat brain stained with ARG44390 anti-RPL18 antibody at 0.5 μg/mL dilution.

  • ARG44390 anti-RPL18 antibody WB image

    Western blot: Mouse testis and Mouse brain stained with ARG44390 anti-RPL18 antibody at 0.5 μg/mL dilution.

  • ARG44390 anti-RPL18 antibody IHC-P image

    Immunohistochemistry: Mouse colon stained with ARG44390 anti-RPL18 antibody at 2 μg/ml dilution.