ARG54926
anti-RIPK3 / RIP3 antibody
anti-RIPK3 / RIP3 antibody for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Signaling Transduction antibody
Overview
Product Description | Rabbit Polyclonal antibody recognizes RIPK3 / RIP3 |
---|---|
Tested Reactivity | Hu, Ms, Rat |
Tested Application | ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | RIPK3 / RIP3 |
Antigen Species | Mouse |
Immunogen | A 14-amino acid peptide within the last 50 amino acids of murine RIPK3 / RIP3. |
Conjugation | Un-conjugated |
Alternate Names | Receptor-interacting serine/threonine-protein kinase 3; Receptor-interacting protein 3; RIP-3; RIP3; RIP-like protein kinase 3; EC 2.7.11.1 |
Application Instructions
Application Suggestion |
|
||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||||||||
Positive Control | A431 and C2C12 | ||||||||||||||
Observed Size | ~ 57 kDa |
Properties
Form | Liquid |
---|---|
Purification | Affinity purification with immunogen. |
Buffer | PBS and 0.02% Sodium azide |
Preservative | 0.02% Sodium azide |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
---|---|
Gene Symbol | Ripk3 |
Gene Full Name | receptor-interacting serine-threonine kinase 3 |
Background | RIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues. |
Function | Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production. [UniProt] |
Highlight | Related Antibody Duos and Panels: ARG30344 Necrosome Antibody Panel Related products: RIPK3 antibodies; RIPK3 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: A non-autophatic role of Atg9a in necrosis and developmental bone formation RIP1 activation and pathogenesis of NASH Ripoptosome & Necrosome antibody panels are launched |
Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Signaling Transduction antibody |
Calculated MW | 57 kDa |
PTM | RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. Phosphorylation of Ser-199 plays a role in the necroptotic function of RIPK3. Phosphorylation at Ser-227 is required for binding MLKL. Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B. |
Images (5) Click the Picture to Zoom In
-
ARG54926 anti-RIPK3 / RIP3 antibody ICC/IF image
Immunofluorescence: Rat kidney cells stained with ARG54926 anti-RIPK3 / RIP3 antibody at 20 µg/ml dilution.
-
ARG54926 anti-RIPK3 / RIP3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney tissue. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG54926 anti-RIPK3 / RIP3 antibody at 2.5 µg/ml dilution, overnight at 4°C. Counter stained with Hematoxylin.
-
ARG54926 anti-RIPK3 / RIP3 antibody WB image
Western blot: 15 µg of A431 cell lysate stained with ARG54926 anti-RIPK3 / RIP3 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
-
ARG54926 anti-RIPK3 / RIP3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG54926 anti-RIPK3 / RIP3 antibody at 5 µg/ml dilution, overnight at 4°C. Counter stained with Hematoxylin.
-
ARG54926 anti-RIPK3 / RIP3 antibody WB image
Western blot: 15 µg of C2C12 cell lysate stained with ARG54926 anti-RIPK3 / RIP3 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.