ARG54926

anti-RIPK3 / RIP3 antibody

anti-RIPK3 / RIP3 antibody for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Signaling Transduction antibody
publication_link Publication2

Overview

Product Description Rabbit Polyclonal antibody recognizes RIPK3 / RIP3
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RIPK3 / RIP3
Antigen Species Mouse
Immunogen A 14-amino acid peptide within the last 50 amino acids of murine RIPK3 / RIP3.
Conjugation Un-conjugated
Alternate Names Receptor-interacting serine/threonine-protein kinase 3; Receptor-interacting protein 3; RIP-3; RIP3; RIP-like protein kinase 3; EC 2.7.11.1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF20 μg/ml
IHC-FrAssay-dependent
IHC-P5 μg/ml
IP20 μg/ml
WB0.1 - 0.5 μg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A431 and C2C12
Observed Size ~ 57 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11035 Human RIPK3

GeneID: 246240 Rat RIPK3

GeneID: 56532 Mouse RIPK3

Gene Symbol Ripk3
Gene Full Name receptor-interacting serine-threonine kinase 3
Background RIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.
Function Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30344 Necrosome Antibody Panel
Related products:
RIPK3 antibodies; RIPK3 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
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Ripoptosome & Necrosome antibody panels are launched
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 57 kDa
PTM RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. Phosphorylation of Ser-199 plays a role in the necroptotic function of RIPK3. Phosphorylation at Ser-227 is required for binding MLKL.
Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B.

Images (5) Click the Picture to Zoom In

  • ARG54926 anti-RIPK3 / RIP3 antibody ICC/IF image

    Immunofluorescence: Rat kidney cells stained with ARG54926 anti-RIPK3 / RIP3 antibody at 20 µg/ml dilution.

  • ARG54926 anti-RIPK3 / RIP3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse kidney tissue. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG54926 anti-RIPK3 / RIP3 antibody at 2.5 µg/ml dilution, overnight at 4°C. Counter stained with Hematoxylin.

  • ARG54926 anti-RIPK3 / RIP3 antibody WB image

    Western blot: 15 µg of A431 cell lysate stained with ARG54926 anti-RIPK3 / RIP3 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.

  • ARG54926 anti-RIPK3 / RIP3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat kidney tissue. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG54926 anti-RIPK3 / RIP3 antibody at 5 µg/ml dilution, overnight at 4°C. Counter stained with Hematoxylin.

  • ARG54926 anti-RIPK3 / RIP3 antibody WB image

    Western blot: 15 µg of C2C12 cell lysate stained with ARG54926 anti-RIPK3 / RIP3 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.

Specific References

PARP inhibitor rescues hearing and hair cell impairment in Cx26-null mice

IHC-Fr, WB / Mouse

Xiaohui Wang et al.
View.,  (2023)

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RIP3/MLKL pathway-regulated necroptosis: A new mechanism of paclitaxel-induced peripheral neuropathy

WB / Rat

Dongyang Ma et al.
J Biochem Mol Toxicol.,  (2021)

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