ARG66476
anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5]
anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Publication7
Overview
| Product Description | Rabbit Monoclonal antibody [YJY-1-5] recognizes RIPK1 / RIP1 phospho (Ser166) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | YJY-1-5 |
| Isotype | IgG |
| Target Name | RIPK1 / RIP1 |
| Antigen Species | Mouse |
| Immunogen | Phosphospecific peptide around Ser166 of Mouse RIPK1 / RIP1. |
| Conjugation | Un-conjugated |
| Alternate Names | Receptor-interacting protein 1; RIP-1; Receptor-interacting serine/threonine-protein kinase 1; RIP; Cell death protein RIP; RIP1; EC 2.7.11.1; Serine/threonine-protein kinase RIP |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Citrate buffer (pH 6.0) at high pressure and temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13546 Human Receptor-interacting serine/threonine-protein kinase 1 Swiss-port # Q60855 Mouse Receptor-interacting serine/threonine-protein kinase 1 |
|---|---|
| Gene Symbol | RIPK1 |
| Gene Full Name | receptor (TNFRSF)-interacting serine-threonine kinase 1 |
| Background | RIPK1 / RIP1 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. [provided by RefSeq, Aug 2017] |
| Function | RIPK1 / RIP1: Serine-threonine kinase which is a key regulator of both cell death and cell survival (PubMed:25459879). Exhibits kinase activity-dependent functions that trigger cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival (PubMed:11101870, PubMed:25459879). Initiates ripoptocide which describes cell death that is dependent on RIPK1, be it apoptosis or necroptosis (PubMed:31457011). Upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kB pathway. Specific conditions can however activate RIPK1, and its kinase activity then regulates assembly of two death-inducing complexes, namely complex IIa (RIPK1-FADD-CASP8) and the complex IIb (RIPK1-RIPK3-MLKL) and these complexes respectively drive apoptosis or necroptosis, a regulated form of necrosis (PubMed:19524513, PubMed:19524512, PubMed:29440439, PubMed:30988283). During embryonic development suppresses apoptosis and necroptosis and prevents the interaction of TRADD with FADD thereby limiting aberrant activation of CASP8. Phosphorylates DAB2IP at 'Ser-728' in a TNF- alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade (PubMed:17389591). Required for ZBP1-induced NF-kappaB activation and activation of NF-kappaB by DNA damage and IR. [UniProt] |
| Cellular Localization | Cytoplasm. Cell membrane. [UniProt] |
| Highlight | Related products: RIPK1 antibodies; RIPK1 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: RIP1 activation and pathogenesis of NASH Ripoptosome & Necrosome antibody panels are launched RIP1 pSer166 antibody good for human and mouse samples |
| Calculated MW | 76 kDa |
| PTM | Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apoptotic signaling through the TRADD-FADD interaction. RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. Phosphorylation of Ser-161 by RIPK3 is necessary for the formation of the necroptosis-inducing complex. Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently down-regulates TNF-alpha-induced NFkappa-B signaling. 'Lys-48'-linked polyubiquitination by RFFL or RNF34 also promotes proteasomal degradation and negatively regulates TNF-alpha-induced NFkappa-B signaling. Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains. Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG66476 anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5] WB image
Western blot: MLE cells untreated control (C) or treated with 20 ng/ml of TNF alpha, 1 μM of BV-6 and 20 μM of Z-VAD-FMK for 8 hours (T). Cell lysates were stained with ARG66476 anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5].
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ARG66476 anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5] IHC-P image
Immunohistochemistry: Paraffin-embedded rat lung tissue from rat silicosis model. Antigen Retrieval: Citrate buffer (pH 6.0) at high pressure and temperature. The tissue section was stained with ARG66476 anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5] at 1:70 dilution. The picture on the right is negative control.
Customer's Feedback
Excellent
Review for anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5]
Application：WB
Sample：MLE cells untreated control (C) or treated with 20 ng/ml of TNF alpha, 1 μM of BV-6 and 20 μM of Z-VAD-FMK for 8 hours (T)
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5]
Application：IHC
Sample：Rat lung from rat silicosis model
Sample Preparation Method：Paraffin
Antigen Retrieval Buffer：Citrate acid buffer
Primary Antibody Dilution Factor：1:75
Excellent
Review for anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5]
Application：IHC
Sample：Rat lung from rat silicosis model
Sample Preparation Method：Paraffin
Antigen Retrieval Buffer：Citrate buffer (pH 6.0)
Primary Antibody Dilution Factor：1:70
Specific References
Inhibition of necroptosis mitigates paclitaxelinduced neuronal damage and cognitive impairment
WB / Mouse
Silencing of TRAF5 enhances necroptosis in hepatocellular carcinoma by inhibiting LTBR-mediated NF-κB signaling
WB / Human
Housing conditions affect enterocyte death mode and turnover rate in mouse small intestine
WB / Mouse
Neuronal MD2 induces long-term mental impairments in septic mice by facilitating necroptosis and apoptosis
WB / Mouse
Pyroptosis and Necroptosis Inhibitor Necrosulfonamide Prevents Lipopolysaccharide-Induced Inflammatory Hyperalgesia in Mice
WB / Mouse
NEK1-mediated retromer trafficking promotes blood-brain barrier integrity by regulating glucose metabolism and RIPK1 activation.
WB / Mouse
RIP1 kinase activity promotes steatohepatitis through mediating cell death and inflammation in macrophages.
WB, IHC-P / Mouse
