ARG41296
anti-RGR antibody
anti-RGR antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RGR |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RGR |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide between aa. 265-291 of Human RGR. |
| Conjugation | Un-conjugated |
| Alternate Names | RP44; RPE-retinal G protein-coupled receptor |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HL-60 | ||||||||
| Observed Size | 32 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P47804 Human RPE-retinal G protein-coupled receptor |
|---|---|
| Gene Symbol | RGR |
| Gene Full Name | retinal G protein coupled receptor |
| Background | This gene encodes a putative retinal G-protein coupled receptor. The gene is a member of the opsin subfamily of the 7 transmembrane, G-protein coupled receptor 1 family. Like other opsins which bind retinaldehyde, it contains a conserved lysine residue in the seventh transmembrane domain. The protein acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. The reverse isomerization occurs with rhodopsin in retinal photoreceptor cells. The protein is exclusively expressed in tissue adjacent to retinal photoreceptor cells, the retinal pigment epithelium and Mueller cells. This gene may be associated with autosomal recessive and autosomal dominant retinitis pigmentosa (arRP and adRP, respectively). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Function | Receptor for all-trans- and 11-cis-retinal. Binds preferentially to the former and may catalyze the isomerization of the chromophore by a retinochrome-like mechanism. [UniProt] |
| Cellular Localization | Membrane; Multi-pass membrane protein. [UniProt] |
| Calculated MW | 32 kDa |
| PTM | Covalently binds all-trans- and 11-cis-retinal. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG41296 anti-RGR antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain tissue stained with ARG41296 anti-RGR antibody.
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ARG41296 anti-RGR antibody WB image
Western blot: 20 ug of HL-60 whole cell lysate stained with ARG41296 anti-RGR antibody at 1:1000 dilution.
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ARG41296 anti-RGR antibody FACS image
Flow Cytometry: HL-60 cells stained with ARG41296 anti-RGR antibody (right histogram) or without primary antibody as control (left histogram), followed by incubation with FITC labelled secondary antibody.
