ARG43142
anti-REA antibody
anti-REA antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes REA |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | REA |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to M1-K299 of Human REA. |
| Conjugation | Un-conjugated |
| Alternate Names | BAP; Bap37; BCAP37; D-prohibitin; Repressor of estrogen receptor activity; Prohibitin-2; PNAS-141; B-cell receptor-associated protein BAP37; p22; hBAP; REA |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 32 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunogen affinity purified. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.01% Sodium azide and 4% Trehalose. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PHB2 |
| Gene Full Name | prohibitin 2 |
| Function | Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. In mitochondria, regulates cytochrome-c oxidase assembly (COX) and mitochondrial respiration. Binding to sphingoid 1-phosphate (SPP) modulates its regulator activity (PubMed:20959514). [UniProt] |
| Cellular Localization | Mitochondrion inner membrane. Cytoplasm. Nucleus. [UniProt] |
| Calculated MW | 33 kDa |
Images (8) Click the Picture to Zoom In
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ARG43142 anti-REA antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG43142 anti-REA antibody (green) at 5 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG43142 anti-REA antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43142 anti-REA antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG43142 anti-REA antibody WB image
Western blot: 50 µg of sample under reducing conditions. HeLa, Jurkat, HEK293, K562, HT1080, SW620, U87 and A549 whole cell lysates stained with ARG43142 anti-REA antibody at 0.25 µg/ml dilution, overnight at 4°C.
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ARG43142 anti-REA antibody FACS image
Flow Cytometry: HL-60 cells were blocked with 10% normal goat serum and then stained with ARG43142 anti-REA antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG43142 anti-REA antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human rectal cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43142 anti-REA antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG43142 anti-REA antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43142 anti-REA antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG43142 anti-REA antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43142 anti-REA antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG43142 anti-REA antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat brain, Rat heart, Rat liver, PC-12, Mouse brain, Mouse heart, Mouse liver, NIH/3T3, Raw264.7 whole cell lysates stained with ARG43142 anti-REA antibody at 0.25 µg/ml dilution, overnight at 4°C.
