ARG23897
anti-Phosphoserine / threonine antibody [M380A + M380B]
anti-Phosphoserine / threonine antibody [M380A + M380B] for ELISA,ICC/IF,Immunoprecipitation,Western blot and Other
Overview
| Product Description | Mouse Monoclonal antibody [M380A + M380B] recognizes Phosphoserine / threonine |
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| Tested Reactivity | Other |
| Tested Application | ELISA, ICC/IF, IP, WB |
| Specificity | This antibody detects many serine or threonine phosphorylated proteins by WB, ICC and ELISA. This product is a mix of two clones: M380A and M380B. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | M380A + M380B |
| Isotype | IgG1 |
| Target Name | Serine / Threonine |
| Antigen Species | Others |
| Immunogen | Clone M380A was generated from a phosphothreonine synthetic peptide (coupled to carrier protein) and Clone M380B was generated from a phosphoserine synthetic peptide (coupled to carrier protein). |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Purification | Purification with Protein A. |
|---|---|
| Buffer | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 50% Glycerol and 1 mg/ml BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity. |
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Images (2) Click the Picture to Zoom In
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ARG23897 anti-Phosphoserine / threonine antibody [M380A + M380B] WB image
Western blot: A431 cells treated with calyculin A (100 nM) for 30 min (lane 1 and 2) then treated with lambda phosphatase (lane 3). The blot was stained with ARG23897 anti-Phosphoserine / threonine antibody [M380A + M380B] at 1:250 (lane 1) or 1:1000 (lanes 2 and 3).
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ARG23897 anti-Phosphoserine / threonine antibody [M380A + M380B] ICC/IF image
Immunofluorescence: Control (left) and calyculin A-treated (right) A431 cells were stained with ARG23897 anti-Phosphoserine / threonine antibody [M380A + M380B].
