ARG42836
anti-PTBP1 / PTB antibody [3H12]
anti-PTBP1 / PTB antibody [3H12] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [3H12] recognizes PTBP1 / PTB |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3H12 |
| Isotype | IgG2b |
| Target Name | PTBP1 / PTB |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to M1-A504 of Human PTBP1 / PTB. |
| Conjugation | Un-conjugated |
| Alternate Names | PTB-1; PTB-T; HNRPI; PTB; PTB3; HNRNPI; Heterogeneous nuclear ribonucleoprotein I; hnRNP I; HNRNP-I; Polypyrimidine tract-binding protein 1; PTB4; PTB2; pPTB; 57 kDa RNA-binding protein PPTB-1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 57 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P26599 Human Polypyrimidine tract-binding protein 1 |
|---|---|
| Gene Symbol | PTBP1 |
| Gene Full Name | polypyrimidine tract binding protein 1 |
| Background | This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Function | Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (PubMed:15009664). In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:21518806). [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 57 kDa |
Images (8) Click the Picture to Zoom In
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ARG42836 anti-PTBP1 / PTB antibody [3H12] ICC/IF image
Immunofluorescence: HeLa cells were blocked with 10% goat serum and then stained with ARG42836 anti-PTBP1 / PTB antibody [3H12] (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG42836 anti-PTBP1 / PTB antibody [3H12] IHC-P image
Immunohistochemistry: Paraffin-embedded Human rectal cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42836 anti-PTBP1 / PTB antibody [3H12] at 1 µg/ml dilution, overnight at 4°C.
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ARG42836 anti-PTBP1 / PTB antibody [3H12] WB image
Western blot: 50 µg of sample under reducing conditions. HeLa, K562, A549, HEK293, Jurkat, Raji, PC-12 and NIH/3T3 whole cell lysates stained with ARG42836 anti-PTBP1 / PTB antibody [3H12] at 0.5 µg/ml dilution, overnight at 4°C.
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ARG42836 anti-PTBP1 / PTB antibody [3H12] FACS image
Flow Cytometry: K562 cells were blocked with 10% normal goat serum and then stained with ARG42836 anti-PTBP1 / PTB antibody [3H12] (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Mouse IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG42836 anti-PTBP1 / PTB antibody [3H12] IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42836 anti-PTBP1 / PTB antibody [3H12] at 1 µg/ml dilution, overnight at 4°C.
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ARG42836 anti-PTBP1 / PTB antibody [3H12] IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42836 anti-PTBP1 / PTB antibody [3H12] at 1 µg/ml dilution, overnight at 4°C.
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ARG42836 anti-PTBP1 / PTB antibody [3H12] IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42836 anti-PTBP1 / PTB antibody [3H12] at 2 µg/ml dilution, overnight at 4°C.
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ARG42836 anti-PTBP1 / PTB antibody [3H12] IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42836 anti-PTBP1 / PTB antibody [3H12] at 2 µg/ml dilution, overnight at 4°C.
