Overview

Product Description	Rabbit Polyclonal antibody recognizes PRMT1
Tested Reactivity	Hu, Ms, Rat
Tested Application	ChIP, ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	PRMT1
Antigen Species	Human
Immunogen	Synthetic peptide of Human PRMT1.
Conjugation	Un-conjugated
Alternate Names	IR1B4; EC 2.1.1.125; ANM1; Protein arginine N-methyltransferase 1; Histone-arginine N-methyltransferase PRMT1; HCP1; EC 2.1.1.-; Interferon receptor 1-bound protein 4; HRMT1L2

Application Instructions

Application Suggestion	Tested Application Dilution ChIP Assay-dependent ICC/IF 1:100 - 1:500 IHC-P 1:50 - 1:200 WB 1:500 - 1:1000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	NIH/3T3
Observed Size	~ 42 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 15469 Mouse PRMT1 GeneID: 3276 Human PRMT1 GeneID: 60421 Rat PRMT1 Swiss-port # Q63009 Rat Protein arginine N-methyltransferase 1 Swiss-port # Q99873 Human Protein arginine N-methyltransferase 1 Swiss-port # Q9JIF0 Mouse Protein arginine N-methyltransferase 1 More
Gene Symbol	PRMT1
Gene Full Name	protein arginine methyltransferase 1
Background	This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]
Function	Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. [UniProt]
Cellular Localization	Nucleus. Nucleus, nucleoplasm. Cytoplasm, cytosol. Note=Mostly found in the cytoplasm. Colocalizes with CHTOP within the nucleus. Low levels detected also in the chromatin fraction (By similarity). [UniProt]
Calculated MW	42 kDa

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