ARG40937
anti-PPP4C antibody
anti-PPP4C antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PPP4C |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PPP4C |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-307 of Human PPP4C (NP_002711.1). |
| Conjugation | Un-conjugated |
| Alternate Names | PPP4; PPX; PP-X; Pp4; PPH3; EC 3.1.3.16; Serine/threonine-protein phosphatase 4 catalytic subunit; PP4; PP4C; Protein phosphatase X |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse spleen | ||||||||
| Observed Size | 36 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PPP4C |
| Gene Full Name | protein phosphatase 4, catalytic subunit |
| Function | Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. [UniProt] |
| Calculated MW | 35 kDa |
| PTM | Methylation at the C-terminal Leu-307 is critical for interactions with regulatory subunits and functions in DNA repair. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG40937 anti-PPP4C antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG40937 anti-PPP4C antibody at 1:100 dilution.
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ARG40937 anti-PPP4C antibody WB image
Western blot: 25 µg of Mouse spleen lysate stained with ARG40937 anti-PPP4C antibody at 1:1000 dilution.
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ARG40937 anti-PPP4C antibody IP image
Immunoprecipitation: 200 µg extracts of 293T cells were immunoprecipitated and stained with ARG40937 anti-PPP4C antibody at 1:1000 dilution.
