ARG55241
anti-PPAR gamma antibody
anti-PPAR gamma antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody
Publication8
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PPAR gamma |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PPAR gamma |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 2-35 (N-terminus) of Human PPAR gamma. |
| Conjugation | Un-conjugated |
| Alternate Names | PPARgamma; PPAR-gamma; GLM1; PPARG2; PPARG1; CIMT1; NR1C3; Nuclear receptor subfamily 1 group C member 3; Peroxisome proliferator-activated receptor gamma |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PPARG |
| Gene Full Name | peroxisome proliferator-activated receptor gamma |
| Background | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Function | Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity). [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. Note=Redistributed from the nucleus to the cytosol through a MAP2K1/MEK1-dependent manner CCRN4L/NOC enhances its nuclear translocation |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody |
| Calculated MW | 58 kDa |
| PTM | O-GlcNAcylation at Thr-84 reduces transcriptional activity in adipocytes. Phosphorylated in basal conditions and dephosphorylated when treated with the ligand. May be dephosphorylated by PPP5C. The phosphorylated form may be inactive and dephosphorylation at Ser-112 induces adipogenic activity (By similarity). |
Images (3) Click the Picture to Zoom In
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ARG55241 anti-PPAR gamma antibody IHC-P image
Immunohistochemistry: Mouse bone stained with ARG55241 anti-PPAR gamma antibody.
From Jingyuan Wen et al. Biomed Pharmacother., (2023) doi: 10.1016/j.biopha.2022.114122, Fig. 5C.
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ARG55241 anti-PPAR gamma antibody WB image
Western blot: 30 µg of MCF7 and MDA-MB-231 (PPAR gamma low expression cell line) stained with ARG55241 anti-PPAR gamma antibody at 1:1000 dilution.
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ARG55241 anti-PPAR gamma antibody WB image
Western blot: 30 µg of Rat liver lysate stained with ARG55241 anti-PPAR gamma antibody at 1:1000 dilution.
Customer's Feedback
Excellent
Review for anti-PPAR gamma antibody
Application：WB
Sample：U87-MG (untreated or treated with cannabinoid)
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：16 hours
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：HRP
Excellent
Review for anti-PPAR gamma antibody
Application：WB
Sample：Rat liver
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-PPAR gamma antibody
Application：WB
Sample：MCF7 and MDA-MB-231 (PPAR gamma low expression cell line)
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Chondroprotective Mechanism of Eucommia ulmoides Oliv.-Glycyrrhiza uralensis Fisch. Couplet Medicines in Knee Osteoarthritis via Experimental Study and Network Pharmacology Analysis
IHC-P / Mouse
Glycyrrhizic acid alters the hyperoxidative stress-induced differentiation commitment of MSCs by activating the Wnt/β-catenin pathway to prevent SONFH
WB / Human
Qiangguyin inhibited fat accumulation in OVX mice through the p38 MAPK signaling pathway to achieve anti-osteoporosis effects
IHC-P / Mouse
Hyperkeratinization and Proinflammatory Cytokine Expression in Meibomian Glands Induced by Staphylococcus aureus
WB, IHC-P / Mouse
Platelet-rich plasma promotes bone formation, restrains adipogenesis and accelerates vascularization to relieve steroids-induced osteonecrosis of the femoral head.
IHC-P / Rat
Organotypic Culture of Mouse Meibomian Gland: A Novel Model to Study Meibomian Gland Dysfunction In Vitro.
IHC-Fr, WB / Mouse
Genome-wide microRNA screening reveals miR-582-5p as a mesenchymal stem cell-specific microRNA in subchondral bone of the human knee joint.
WB / Human WB / Mouse
Upregulation of transient receptor potential melastatin 6 channel expression by rosiglitazone and all-trans-retinoic acid in erlotinib-treated renal tubular epithelial cells.
WB / IF / Rat
