ARG55240
anti-PPAR alpha antibody
anti-PPAR alpha antibody for Flow cytometry,ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody
Publication3
Overview
| Product Description | Mouse Monoclonal antibody recognizes PPAR alpha |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Target Name | PPAR alpha |
| Antigen Species | Human |
| Immunogen | Recombination protein of Human PPAR alpha (Swiss: Q07869). |
| Conjugation | Un-conjugated |
| Alternate Names | hPPAR; NR1C1; Peroxisome proliferator-activated receptor alpha; PPAR; Nuclear receptor subfamily 1 group C member 1; PPAR-alpha; PPARalpha |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PPARA |
| Gene Full Name | peroxisome proliferator-activated receptor alpha |
| Background | Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008] |
| Function | Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2. [UniProt] |
| Cellular Localization | Nucleus. |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody |
| Calculated MW | 52 kDa |
Images (3) Click the Picture to Zoom In
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ARG55240 anti-PPAR alpha antibody WB image
Western blot: 30 μg of HepG2, HEK293T, HCT116, NIH-3T3, Mouse brain, Rat brain and Rat ovary lysates stained with ARG55240 anti-PPAR alpha antibody at 1:500 dilution.
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ARG55240 anti-PPAR alpha antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG55240 anti-PPAR alpha antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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ARG55240 anti-PPAR alpha antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue stained with ARG55240 anti-PPAR alpha antibody at 1:25 dilution.
Specific References
Diet suppresses glioblastoma initiation in mice by maintaining quiescence of mutation-bearing neural stem cells
ICC/IF / Mouse
PCSK9Qβ-003 Vaccine Attenuates Atherosclerosis in Apolipoprotein E-Deficient Mice.
WB / Mouse
Vaccine Against PCSK9 Improved Renal Fibrosis by Regulating Fatty Acid β-Oxidation.
WB / Mouse
