ARG63767
anti-POMC / Proopiomelanocortin antibody
anti-POMC / Proopiomelanocortin antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes POMC / Proopiomelanocortin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Specificity | Both transcript variants encode the same protein. In Western blot, this product appears to react with the full length precursor protein only. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | POMC / Proopiomelanocortin |
| Antigen Species | Human |
| Immunogen | C-NAIIKNAYKKGE |
| Conjugation | Un-conjugated |
| Alternate Names | Alpha-MSH; Beta-MSH; CLIP; Gamma-MSH; LPH; Corticotropin-lipotropin; NPP; ACTH; POMC; Gamma-LPH; Adrenocorticotropic hormone; MSH; Beta-LPH; Pro-opiomelanocortin; POC |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 29 kDa |
| PTM | Specific enzymatic cleavages at paired basic residues yield the different active peptides. O-glycosylated; reducing sugar is probably N-acetylgalactosamine. |
Images (3) Click the Picture to Zoom In
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ARG63767 anti-POMC / Proopiomelanocortin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human pituitary gland tissue. Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG63767 anti-POMC / Proopiomelanocortin antibody at 2 µg/ml dilution followed by HRP-staining.
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ARG63767 anti-POMC / Proopiomelanocortin antibody WB image
Western blot: 35 µg of NIH/3T3 (A) and Rat brain (B) lysates (in RIPA buffer) stained with ARG63767 anti-POMC / Proopiomelanocortin antibody at 0.3 µg/ml (A) and 2 µg/ml (B) dilutions and incubated at RT for 1 hour.
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ARG63767 anti-POMC / Proopiomelanocortin antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed A431 cells permeabilized with 0.5% Triton. Cells were stained with ARG63767 anti-POMC / Proopiomelanocortin antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
