ARG65754

anti-PMCA2 antibody

anti-PMCA2 antibody for Western blot and Human,Mouse,Rat

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Overview

Product Description Rabbit Polyclonal antibody recognizes PMCA2
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PMCA2
Antigen Species Human
Immunogen Recombinant protein of Human PMCA2
Conjugation Un-conjugated
Alternate Names PMCA2; PMCA2i; PMCA2a; EC 3.6.3.8; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium pump isoform 2; Plasma membrane calcium-transporting ATPase 2

Application Instructions

Predict Reactivity Note Human
Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse liver

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11941 Mouse ATP2B2

GeneID: 24215 Rat ATP2B2

GeneID: 491 Human ATP2B2

Gene Symbol ATP2B2
Gene Full Name ATPase, Ca++ transporting, plasma membrane 2
Background The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Function This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. [UniProt]
Calculated MW 137 kDa

Images (1) Click the Picture to Zoom In

  • ARG65754 anti-PMCA2 antibody WB image

    Western blot: Mouse liver lysate stained with ARG65754 anti-PMCA2 antibody.

Customer's Feedback

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Review for anti-PMCA2 antibody

Application:WB

Sample:Mouse lung

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC