ARG55901
anti-PKM2 antibody
anti-PKM2 antibody for ICC/IF,Western blot and Human,Mouse,Rat,Monkey
Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Colorectal Carcinoma Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes PKM2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | PKM2 |
| Antigen Species | Human |
| Immunogen | Purified recombinant Human PKM2 protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | PK3; PKM2; OIP3; Pyruvate kinase muscle isozyme; CTHBP; HEL-S-30; THBP1; OIP-3; Pyruvate kinase 2/3; Tumor M2-PK; Cytosolic thyroid hormone-binding protein; EC 2.7.1.40; Opa-interacting protein 3; p58; TCB; Pyruvate kinase PKM; Thyroid hormone-binding protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.03% Proclin-300 and 50% Glycerol |
| Preservative | 0.03% Proclin-300 |
| Stabilizer | 50% Glycerol |
| Concentration | 2.6 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PKM |
| Gene Full Name | pyruvate kinase, muscle |
| Background | This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011] |
| Function | Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. [UniProt] |
| Cellular Localization | Cytoplasm; Nucleus |
| Research Area | Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Colorectal Carcinoma Marker antibody |
| Calculated MW | 58 kDa |
| PTM | ISGylated. Under hypoxia, hydroxylated by EGLN3. Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy. FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35. |
Images (2) Click the Picture to Zoom In
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ARG55901 anti-PKM2 antibody ICC/IF image
Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG55901 anti-PKM2 antibody at 1:400 dilution.
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ARG55901 anti-PKM2 antibody WB image
Western blot: 3T3 cell lysate stained with ARG55901 anti-PKM2 antibody at 1:1000 dilution.
