ARG62605
anti-PCNA antibody [PC10]
anti-PCNA antibody [PC10] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Chicken,D. melanogaster ,Monkey,Pigeon,Pig,Zebrafish
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody
Publication9
Overview
| Product Description | Mouse Monoclonal antibody [PC10] recognizes PCNA |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Chk, Dm, Mk, Pgn, Pig, Zfsh |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PC10 |
| Isotype | IgG2a |
| Target Name | PCNA |
| Antigen Species | Rat |
| Immunogen | Protein A-rat PCNA (proliferating cell nuclear antigen) fusion obtained from pC2T |
| Conjugation | Un-conjugated |
| Alternate Names | PCNA; ATLD2; Cyclin; Proliferating cell nuclear antigen |
Application Instructions
| Application Suggestion |
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| Application Note | ICC/IF: Methanol fixed samples. IHC-P: Antigen Retrieval: Heat mediated in Citrate buffer (pH 6.0) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | HEK293 |
Properties
| Form | Liquid |
|---|---|
| Buffer | PBS (pH 7.6), 0.02% Sodium azide and 0.4M arginine. |
| Preservative | 0.02% Sodium azide |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Pcna |
| Gene Full Name | proliferating cell nuclear antigen |
| Background | The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008] |
| Function | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (By similarity). [UniProt] |
| Cellular Localization | Nucleus. Note: Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. [uniprot: P12004] |
| Highlight | Related products: PCNA antibodies; PCNA Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody |
| Calculated MW | 29 kDa |
| PTM | Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA. Acetylated by CREBBP and p300/EP300; preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation (PubMed:24939902, PubMed:19419956). Lysine acetylation disrupts association with chromatin, hence promoting PCNA ubiquitination and proteasomal degradation in response to UV damage in a CREBBP- and EP300-dependent manner (PubMed:24939902). Acetylation disrupts interaction with NUDT15 and promotes degradation (PubMed:19419956). Ubiquitinated (PubMed:24939902, PubMed:20227374). Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase. Methylated on glutamate residues by ARMT1/C6orf211. |
Images (2) Click the Picture to Zoom In
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ARG62605 anti-PCNA antibody [PC10] WB image
Western blot: A549, H1299, HCT116 and HepG2 cell lysates stained with ARG62605 anti-PCNA antibody [PC10] at 0.2 µg/ml.
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ARG62605 anti-PCNA antibody [PC10] IHC-P image
Immunohistochemistry: formalin-fixed, paraffin-embedded human breast carcinoma tissue stained with PCNA antibody [PC10] (ARG62605).
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Review for anti-PCNA antibody [PC10]
Application：WB
Sample：293T
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
E2F1/CKS2/PTEN signaling axis regulates malignant phenotypes in pediatric retinoblastoma
WB / Human
Downregulation of AKT/mTOR signaling pathway for Salmonella-mediated autophagy in human anaplastic thyroid cancer
IHC-P / Mouse
Mesenchymal Stem Cells-Derived Exosomes Ameliorate Ischemia/Reperfusion Induced Acute Kidney Injury in a Porcine Model
WB / Pig
Single-cell analyses unravel cell type-specific responses to a vitamin D analog in prostatic precancerous lesions.
IHC-P / Mouse
Protein arginine methyltransferase 1 mediates renal fibroblast activation and fibrogenesis through activation of Smad3 signaling.
IHC-P / Rat
Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma.
IHC-P / Human
Discoidin domain receptor 1 regulates endochondral ossification through terminal differentiation of chondrocytes.
IHC-P / Mouse
CSN6 expression is associated with pancreatic cancer progression and predicts poor prognosis.
WB, IHC-P / Human
STAT1-mediated inhibition of FOXM1 enhances gemcitabine sensitivity in pancreatic cancer.
IHC-P / Human
