ARG66992
anti-Occludin antibody
anti-Occludin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Occludin |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Occludin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to C-terminus of human occludin. |
| Conjugation | Un-conjugated |
| Alternate Names | PPP1R115; BLCPMG; Occludin |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 60 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 60-70 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300, 1%BSA and 20% Glycerol. |
| Preservative | 0.025% ProClin 300 |
| Stabilizer | 1%BSA and 20% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | OCLN |
| Gene Full Name | occludin |
| Background | This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011] |
| Function | May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. [UniProt] |
| Cellular Localization | Membrane |
| Highlight | Related products: Occludin antibodies; Occludin Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 59 kDa |
| PTM | Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1. |
Images (4) Click the Picture to Zoom In
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ARG66992 anti-Occludin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded mouse tissue stained with ARG66992 anti-Occludin antibody.
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ARG66992 anti-Occludin antibody WB image
Western blot: HepG2 and A549 stained with ARG66992 anti-Occludin antibody at 7.5% SDS-PAGE, 1:1000 dilution.
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ARG66992 anti-Occludin antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded prostate cancer tissue stained with ARG66992 anti-Occludin antibody at 1:100 dilution.. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 6.0.
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ARG66992 anti-Occludin antibody WB image
Western blot: Mouse kidney and mouse testis stained with ARG66992 anti-Occludin antibody at 7.5% SDS-PAGE, 1:1000 dilution.
