ARG52375
anti-Nuclear pore Complex antibody [39C7]
anti-Nuclear pore Complex antibody [39C7] for ICC/IF,Western blot and Human,Mouse
Controls and Markers antibody; Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [39C7] recognizes Nuclear pore Complex |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 39C7 |
| Isotype | IgG1 |
| Target Name | Nuclear pore Complex |
| Immunogen | Yeast nuclear preparations. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Unpurified |
| Buffer | Tissue culture supernatant. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | Nuclear stain of multiple gene products including Nup62, Nup133 |
|---|---|
| Background | The nuclear pore complex (NPC) is a very large structure made up of at least 50 different proteins that span the double membrane of the nuclear envelope functioning as a gateway for macromolecular traffic between the cytoplasm and the nucleus (Nakielny and Dreyfuss,1999). Discrete nuclear pore complex proteins or nucleoporins such as NUP98, NUP180 and p62 have been implicated in autoimmune disease and cancer. Patients with primary biliary cirrhosis (PBC) frequently produce autoantibodies against p62 and NUP180 (Wilken et al., 1993; Nesher et al., 2001) while NUP98 translocations have been found in patients with acute myelogenous leukemia (AML) (Jaju et al. 2001). |
| Research Area | Controls and Markers antibody; Gene Regulation antibody |
Images (3) Click the Picture to Zoom In
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ARG52375 anti-Nuclear pore Complex antibody [39C7] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG52375 anti-Nuclear pore Complex antibody [39C7] (red) at 1:100 dilution and costained with ARG52468 anti-Vimentin antibody (green) at 1:10000 dilution. DAPI (blue) for nuclear staining.
Clone 39C7 reveals strong granular staining of the nuclei corresponding to the nuclear pore complex, while the vimentin antibody specifically labels intermediate filaments in these cells.
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ARG52375 anti-Nuclear pore Complex antibody [39C7] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG52375 anti-Nuclear pore Complex antibody [39C7] (green), and ARG52468 chicken anti-vimentin (red).
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ARG52375 anti-Nuclear pore Complex antibody [39C7] WB image
Western blot: HEK293 (cytosol), HEK293 (nuclear), NIH/3T3 (cytosol), NIH/3T3 (nuclear), HeLa (cytosol) and HeLa (nuclear) lysates stained with ARG52375 anti-Nuclear pore Complex antibody [39C7] (green) at 1:100 dilution.
Clone References
Early remodeling of perinuclear Ca2+ stores and nucleoplasmic Ca2+ signaling during the development of hypertrophy and heart failure.
