ARG52348
anti-Neurofilament NF-L antibody [DA2]
anti-Neurofilament NF-L antibody [DA2] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody
Overview
| Product Description | Mouse Monoclonal antibody [DA2] recognizes Neurofilament NF-L |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Chk |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | DA2 |
| Isotype | IgG1 |
| Target Name | Neurofilament NF-L |
| Antigen Species | Pig |
| Immunogen | Preparation of porcine NF-L |
| Epitope | Epitope is likely centered on the HVQEEQIEVE peptide. |
| Conjugation | Un-conjugated |
| Alternate Names | Neurofilament triplet L protein; 68 kDa neurofilament protein; CMT1F; NF68; NFL; CMT2E; Neurofilament light polypeptide; NF-L; PPP1R110 |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~68k Neurofilament L protein. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Total IgG fraction |
| Buffer | Total IgG fraction and 10 mM Sodium azide |
| Preservative | 10 mM Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H . NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease . |
| Highlight | Related Antibody Duos and Panels: ARG30010 Neuronal Cytoskeletal Antibody Duo (NF-L, TUBBIII) ARG30011 Neurofilament Antibody Duo (NF-H, NF-L) ARG30145 Intermediate Neurofilament Antibody Panel (NF-L, NF-M,NF-H) Related products: Neurofilament antibodies; Neurofilament ELISA Kits; Neurofilament Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody |
| Calculated MW | 62 kDa |
| PTM | O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1. |
Images (4) Click the Picture to Zoom In
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ARG52348 anti-Neurofilament NF-L antibody [DA2] WB image
Western blot: 30 µg of Mouse brain lysate stained with ARG52348 anti-Neurofilament NF-L antibody [DA2] at 1:1000 dilution.
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ARG52348 anti-Neurofilament NF-L antibody [DA2] WB image
Western blot: rat cortex lysate stained with ARG52348 anti-Neurofilament NF-L antibody [DA2] showing specific immunolableing of the ~ 68k NF-L protein.
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ARG52348 anti-Neurofilament NF-L antibody [DA2] IHC-Fr image
Immunohistochemistry: Frozen section of Rat frontal cortex tissue stained with ARG52348 anti-Neurofilament NF-L antibody [DA2] (red) at 1:500 dilution, and costained with anti-GFAP antibody (green) at 1:5000 dilution. Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.
Clone DA2 labels cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the GFAP antibody stains the network of glial cells.
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ARG52348 anti-Neurofilament NF-L antibody [DA2] WB image
Western blot: Rat brain, Rat spinal cord, Mouse brain and Mouse spinal cord lysates stained with ARG52348 anti-Neurofilament NF-L antibody [DA2] (green) at 1:5000 dilution.
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Review for anti-Neurofilament NF-L antibody [DA2]
Application：WB
Sample：Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Clone References
Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
WB / Human
Compartmentation of alpha-internexin and neurofilament triplet proteins in cultured hippocampal neurons.
WB / Rat
Identification of a tektin-like protein associated with neurofilaments in the developing chick nervous system.
ICC/IF / Chicken
