ARG52345
anti-Nestin antibody [4D11]
anti-Nestin antibody [4D11] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody
Publication4
Overview
| Product Description | Mouse Monoclonal antibody [4D11] recognizes Nestin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Chk, Cow, Hrs, Pig |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4D11 |
| Isotype | IgG1 |
| Target Name | Nestin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around aa. 317-630 of Human Nestin. |
| Conjugation | Un-conjugated |
| Alternate Names | NES; Nestin FLJ21841; Nbla00170 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | Specific for the ~220-240 kDa nestin doublet in Western blot in neonatal Rat brain homogenate. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Ascites fluid |
| Buffer | Ascites fluid and 0.01 % Sodium azide |
| Preservative | 0.01 % Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NES |
| Gene Full Name | nestin |
| Background | This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. |
| Function | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| Cellular Localization | Intermediate filament |
| Highlight | Related Antibody Duos and Panels: ARG30006 NSC and Astrocyte Marker Antibody Duo (GFAP, Nestin) ARG30008 Neuron Development Marker Antibody Duo (Nestin, Tuj1) ARG30009 NSC and Neuron Marker Antibody Duo (Nestin, MAP2) ARG30146 Neural Stem / Progenitor Cell Marker Antibody Duo (Nestin, Vimentin) Related products: Nestin antibodies; Nestin Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: 14-3-3η as a promising target for the treatment of Major Depression Disorder Stem cell and the regenerative medicine: Ready for the patients Neuronal Development Marker Neuronal Development Marker Choose the Best ZIKA Virus Antibodies Fight microcephaly with arigo |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody |
| Calculated MW | 177 kDa |
| PTM | Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation |
Images (4) Click the Picture to Zoom In
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ARG52345 anti-Nestin antibody [4D11] ICC/IF image
Immunofluorescence: Cultured neonatal Rat neurons and glia showing nestin labeling in red (ARG52345 Nestin antibody [4D11]) and vimentin (ARG52468 anti-Vimentin antibody) in green.
Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
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ARG52345 anti-Nestin antibody [4D11] ICC/IF image
Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG52345 anti-Nestin antibody [4D11] (red) at 1:500 dilution and costained with ARG52328 anti-MAP2 antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining.
The Nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
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ARG52345 anti-Nestin antibody [4D11] WB image
Western blot: Rat brain (P18) homogenate stained with ARG52345 anti-Nestin antibody [4D11].
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ARG52345 anti-Nestin antibody [4D11] WB image
Western blot: Rat brain (embryonic, E18), C6 and SH-SY5Y cell lysates stained with ARG52345 anti-Nestin antibody [4D11] (green) at 1:500 dilution.
Specific References
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ICC/IF / Human
Insulin-like growth factor reduced against decabromodiphenyl ether-209-induced neurodevelopmental toxicity in vivo and in vitro
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Mesenchymal Stem Cell-Derived Exosomes Ameliorate Alzheimer's Disease Pathology and Improve Cognitive Deficits.
WB / Human
Transplantation of encapsulated human Leydig-like cells: A novel option for the treatment of testosterone deficiency.
ICC/IF / Human
