ARG56141
anti-Nav1.8 Na+ Channel antibody [S134-12]
anti-Nav1.8 Na+ Channel antibody [S134-12] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [S134-12] recognizes Nav1.8 Na+ Channel |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | This antibody recognizes Human, Mouse, and Rat Nav1.8. It does not cross-react with other Nav channels. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | S134-12 |
| Isotype | IgG2a |
| Target Name | Nav1.8 Na+ Channel |
| Antigen Species | Rat |
| Immunogen | Fusion protein around aa. 1724-1956 (cytoplasmic C-terminus) of Rat Nav1.8 |
| Conjugation | Un-conjugated |
| Alternate Names | Voltage-gated sodium channel subunit alpha Nav1.8; Nav1.8; PN3; Sodium channel protein type X subunit alpha; FEPS2; SNS; Sodium channel protein type 10 subunit alpha; Peripheral nerve sodium channel 3; hPN3 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Rat dorsal root ganglia or lysate of COS cells transiently expressing Nav1.8. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SCN10A |
| Gene Full Name | sodium channel, voltage gated, type X alpha subunit |
| Background | The protein encoded by this gene is a tetrodotoxin-resistant voltage-gated sodium channel alpha subunit. The properties of the channel formed by the encoded transmembrane protein can be altered by interaction with different beta subunits. This protein may be involved in the onset of pain associated with peripheral neuropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] |
| Function | Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms. [UniProt] |
| Calculated MW | 221 kDa |
| PTM | Ubiquitinated by NEDD4L; which promotes its endocytosis. Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents. Lacks the cysteine which covalently binds the conotoxin GVIIJ. This cysteine (position 816) is speculated in other sodium channel subunits alpha to be implied in covalent binding with the sodium channel subunit beta-2 or beta-4. |
Images (2) Click the Picture to Zoom In
-
ARG56141 anti-Nav1.8 Na+ Channel antibody [S134-12] WB image
Western blot: 20 µg of Mouse brain lysate stained with ARG56141 anti-Nav1.8 Na+ Channel antibody [S134-12] at 1:500 dilution.
-
ARG56141 anti-Nav1.8 Na+ Channel antibody [S134-12] IF image
Immunofluorescence: Rat dorsal root ganglia cryosections stained with ARG56141 anti-Nav1.8 Na+ Channel antibody [S134-12].
Customer's Feedback
Excellent
Review for anti-Nav1.8 Na+ Channel antibody [S134-12]
Application：WB
Sample：Mouse brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
