ARG55254
anti-NOX4 antibody
anti-NOX4 antibody for ELISA,Immunohistochemistry,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Publication3
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NOX4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, IHC, WB |
| Specificity | NOX4 antibody is human, mouse, and rat reactive. At least four isoforms of NOX4 are known to exist. NOX4 is predicted to not cross-react with other NOX proteins. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NOX4 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (14 aa) within aa. 70-120 of Human NOX4. |
| Conjugation | Un-conjugated |
| Alternate Names | RENOX; KOX-1; P; Renal NAD; Kidney oxidase-1; NADPH oxidase 4; KOX; EC 1.6.3.-; Kidney superoxide-producing NADPH oxidase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Jurkat Cell Lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NOX4 |
| Gene Full Name | NADPH oxidase 4 |
| Background | The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide. NOX4 is is expressed in multiple tissues and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). Unlike other NOX proteins, NOX4 does not require cytosolic subunits and thus is constitutively active. The function of NOX4 remains unclear as it plays both protective and deleterious roles in cellular metabolism. |
| Function | Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity. Isoform 4: Involved in redox signaling in vascular cells. Constitutively and NADPH-dependently generates reactive oxygen species (ROS). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Displays an increased activity relative to isoform 1. [UniProt] |
| Highlight | Related products: NOX4 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Pericytes, new therapeutic target for Alzheimer's disease? |
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 67 kDa |
| PTM | Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function. |
Images (4) Click the Picture to Zoom In
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ARG55254 anti-NOX4 antibody WB image
Western blot: 30 µg of Jurkat cell lysate stained with ARG55254 anti-NOX4 antibody at 1:500 dilution.
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ARG55254 anti-NOX4 antibody IHC image
Immunohistochemistry: NOX4 in human spleen tissue stained with ARG55254 anti-NOX4 antibody at 20 μg/ml dilution.
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ARG55254 anti-NOX4 antibody IHC image
Immunohistochemistry: NOX4 in human spleen tissue stained with ARG55254 anti-NOX4 antibody at 5 μg/ml dilution.
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ARG55254 anti-NOX4 antibody WB image
Western blot: Mouse heart stained with ARG55254 anti-NOX4 antibody.
From Lei Zhang et al. Front Physiol (2022), doi: 10.3389/fphys.2022.862187, Fig. 4. C.
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Review for anti-NOX4 antibody
Application：WB
Sample：293T and Jurkat
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Pterostilbene Interferes With Lipopolysaccharide-Induced Myocardial Injury Through Oxidative Stress and Inflammasome Pathways
WB / Mouse
Neohesperidin Protects Angiotensin II-Induced Hypertension and Vascular Remodeling.
WB / Mouse
Treatment effects of the traditional Chinese medicine Shenks in bleomycin-induced lung fibrosis through regulation of TGF-beta/Smad3 signaling and oxidative stress.
IHC-P / Mouse
