ARG57502
anti-NME2 antibody [2E3]
anti-NME2 antibody [2E3] for ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [2E3] recognizes NME2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2E3 |
| Isotype | IgG1, kappa |
| Target Name | NME2 |
| Antigen Species | Human |
| Immunogen | Recombinant Human NME2 (aa. 1-152) purified from E. coli. |
| Conjugation | Un-conjugated |
| Alternate Names | Nucleoside diphosphate kinase B; NDPK-B; NDP kinase B; NDKB; NDPKB; EC 2.7.13.3; PUF; Histidine protein kinase NDKB; NDK B; EC 2.7.4.6; NM23B; C-myc purine-binding transcription factor PUF; NM23-H2; nm23-H2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NME2 |
| Gene Full Name | NME/NM23 nucleoside diphosphate kinase 2 |
| Background | Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010] |
| Function | Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically. Exhibits histidine protein kinase activity. [UniProt] |
| Calculated MW | 17 kDa |
Images (3) Click the Picture to Zoom In
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ARG57502 anti-NME2 antibody [2E3] ICC/IF image
Immunoflorescense: HeLa cells stained with ARG57502 anti-NME2 antibody [2E3] at 1:100 dilution. The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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ARG57502 anti-NME2 antibody [2E3] WB image
Western blot: 40 µg of HeLa, A549, MCF7 and LnCap cell lysates stained with ARG57502 anti-NME2 antibody [2E3] at 1:1000 dilution.
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ARG57502 anti-NME2 antibody [2E3] WB image
Western blot: 5 µg of 293T and NME2 transfected 293T cell lysates stained with ARG57502 anti-NME2 antibody [2E3] at 1:1000 dilution.
