ARG52360
anti-NMDAR2A antibody, N-terminal
anti-NMDAR2A antibody, N-terminal for ICC/IF,IHC-Frozen sections,Western blot and Mouse,Rat
Neuroscience antibody; Postsynaptic Receptor antibody
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NMDAR2A |
|---|---|
| Tested Reactivity | Ms, Rat |
| Predict Reactivity | Bov, Dog |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NMDAR2A |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide corresponding to amino acid residues from the N-terminal region of the NR2A subunit conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | FESD; NR2A; GluN2A; Glutamate receptor ionotropic, NMDA 2A; N-methyl D-aspartate receptor subtype 2A; EPND; Glutamate [NMDA] receptor subunit epsilon-1; NMDAR2A; LKS; hNR2A |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~180k NR2A subunit of the NMDA receptor. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P35436 Mouse Glutamate receptor ionotropic, NMDA 2A Swiss-port # Q00959 Rat Glutamate receptor ionotropic, NMDA 2A |
|---|---|
| Gene Symbol | GRIN2A |
| Gene Full Name | glutamate receptor, ionotropic, N-methyl D-aspartate 2A |
| Background | The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. |
| Research Area | Neuroscience antibody; Postsynaptic Receptor antibody |
| Calculated MW | 165 kDa |
Images (1) Click the Picture to Zoom In
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ARG52360 anti-NMDAR2A antibody, N-terminal WB image
Western Blot: 10 μg of rat hippocampal lysate showing specific immunolabeling of the ~ 180k NR2A subunit stained with NMDAR2A antibody (ARG52360)
Specific References
Involvement of NMDAR/PSD-95/nNOS-NO-cGMP pathway in embryonic exposure to BPA induced learning and memory dysfunction of rats.
WB / Rat
