ARG44095

anti-NIT2 antibody

anti-NIT2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal recognizes NIT2
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NIT2
Antigen Species Human
Immunogen Human NIT2 recombinant protein (Position: M1-K249).
Conjugation Un-conjugated
Alternate Names NIT2; Nitrilase Family Member 2; Omega-Amidase NIT2; Nitrilase Homolog 2; Epididymis Secretory Sperm Binding Protein Li 8a; Nitrilase Family, Member 2; Nit Protein 2; EC 3.5.1.3; HEL-S-8a

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
IHC-P2 - 5 µg/ml
WB0.25 - 0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 288174 Rat NIT2

GeneID: 52633 Mouse NIT2

GeneID: 56954 Human NIT2

Gene Symbol NIT2
Gene Full Name Nitrilase Family Member 2
Background Enables omega-amidase activity. Involved in asparagine metabolic process; glutamine metabolic process; and oxaloacetate metabolic process. Located in centrosome and cytosol.
Function The role of omega-amidase is to remove potentially toxic intermediates by converting 2-oxoglutaramate and 2-oxosuccinamate to biologically useful 2-oxoglutarate and oxaloacetate, respectively.
Cellular Localization Cytoplasm
Calculated MW 30 kDa
PTM Acetylation, Phosphoprotein

Images (6) Click the Picture to Zoom In

  • ARG44095 anti-NIT2 antibody IHC-P image

    Immunohistochemistry: Human squamous cell carcinoma penis stained with ARG44095 anti-NIT2 antibody at 2 μg/ml dilution.

  • ARG44095 anti-NIT2 antibody WB image

    Western blot: A549 stained with ARG44095 anti-NIT2 antibody at 0.5 μg/mL dilution.

  • ARG44095 anti-NIT2 antibody FACS image

    Flow Cytometry: A549 stained with ARG44095 anti-NIT2 antibody at 1 µg/10^6 cells dilution.

  • ARG44095 anti-NIT2 antibody IHC-P image

    Immunohistochemistry: Rat kidney stained with ARG44095 anti-NIT2 antibody at 2 μg/ml dilution.

  • ARG44095 anti-NIT2 antibody WB image

    Western blot: Rat liver and Rat kidney stained with ARG44095 anti-NIT2 antibody at 0.5 μg/mL dilution.

  • ARG44095 anti-NIT2 antibody IHC-P image

    Immunohistochemistry: Mouse kidney stained with ARG44095 anti-NIT2 antibody at 2 μg/ml dilution.