ARG51518

anti-NFkB p65 phospho (Ser536) antibody

anti-NFkB p65 phospho (Ser536) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody; NFkB nuclear translocation Study antibody; Inflammation Study antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes NFkB p65 phospho (Ser536)
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NFkB p65
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 536 (F-S-S(p)-I-A) derived from Human NFκB-p65.
Conjugation Un-conjugated
Alternate Names Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; NFKB3; p65; Transcription factor p65

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:300
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19697 Mouse RELA

GeneID: 5970 Human RELA

Swiss-port # Q04206 Human Transcription factor p65

Swiss-port # Q04207 Mouse Transcription factor p65

Gene Symbol RELA
Gene Full Name v-rel avian reticuloendotheliosis viral oncogene homolog A
Background NFkB is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Function NFkB is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30248 Phospho NFkB Antibody Duo (Total, pS536)
ARG30323 Inflammation Antibody Panel
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NFkB p65 antibodies; NFkB p65 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody; NFkB nuclear translocation Study antibody; Inflammation Study antibody
Calculated MW 60 kDa
PTM Ubiquitinated, leading to its proteasomal degradation. Degradation is required for termination of NF-kappa-B response.
Monomethylated at Lys-310 by SETD6. Monomethylation at Lys-310 is recognized by the ANK repeats of EHMT1 and promotes the formation of repressed chromatin at target genes, leading to down-regulation of NF-kappa-B transcription factor activity. Phosphorylation at Ser-311 disrupts the interaction with EHMT1 without preventing monomethylation at Lys-310 and relieves the repression of target genes (By similarity).
Phosphorylation at Ser-311 disrupts the interaction with EHMT1 and promotes transcription factor activity (By similarity). Phosphorylation on Ser-536 stimulates acetylation on Lys-310 and interaction with CBP; the phosphorylated and acetylated forms show enhanced transcriptional activity. Phosphorylation at Ser-276 by RPS6KA4 and RPS6KA5 promotes its transactivation and transcriptional activities.
Reversibly acetylated; the acetylation seems to be mediated by CBP, the deacetylation by HDAC3 and SIRT2. Acetylation at Lys-122 enhances DNA binding and impairs association with NFKBIA. Acetylation at Lys-310 is required for full transcriptional activity in the absence of effects on DNA binding and NFKBIA association. Acetylation at Lys-310 promotes interaction with BRD4. Acetylation can also lower DNA-binding and results in nuclear export. Interaction with BRMS1 promotes deacetylation of Lys-310. Lys-310 is deacetylated by SIRT2.
S-nitrosylation of Cys-38 inactivates the enzyme activity.
Sulfhydration at Cys-38 mediates the anti-apoptotic activity by promoting the interaction with RPS3 and activating the transcription factor activity.
Sumoylation by PIAS3 negatively regulates DNA-bound activated NF-kappa-B.
Proteolytically cleaved within a conserved N-terminus region required for base-specific contact with DNA in a CPEN1-mediated manner, and hence inhibits NF-kappa-B transcriptional activity (PubMed:18212740).

Images (8) Click the Picture to Zoom In

  • ARG51518 anti-NFkB p65 phospho (Ser536) antibody IHC-P image

    Immunohistochemistry: Rat femoral head stained with ARG51518 anti-NFkB p65 phospho (Ser536) antibody at 1:300 dilution.

    From Huihui Xu et al. Apoptosis. (2023), doi: 10.1007/s10495-023-01860-2, Fig. 6A.

  • ARG51518 anti-NFkB p65 phospho (Ser536) antibody IHC-P image

    Immunohistochemistry: Mouse tibial cartilage stained with ARG51518 anti-NFkB p65 phospho (Ser536) antibody.

    From Congzi Wu et al. Biomed Res Int. (2022), doi: 10.1155/2022/9230784, Fig. 6. c.

  • ARG51518 anti-NFkB p65 phospho (Ser536) antibody WB image

    Western blot: 20 µg of HeLa cells untreated or treated with TNF-alpha at 10, 20 or 30 min. The blots were stained with ARG51518 anti-NFkB p65 phospho (Ser536) antibody at 1:500 dilution.

  • ARG51518 anti-NFkB p65 phospho (Ser536) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51518 anti-NFkB p65 phospho (Ser536) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51518 anti-NFkB p65 phospho (Ser536) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with ARG51518 anti-NFkB p65 phospho (Ser536) antibody.

  • ARG51518 anti-NFkB p65 phospho (Ser536) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51518 anti-NFkB p65 phospho (Ser536) antibody.

  • ARG51518 anti-NFkB p65 phospho (Ser536) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human Lung carcinoma tissue stained with ARG51518 anti-NFkB p65 phospho (Ser536) antibody.

  • ARG51518 anti-NFkB p65 phospho (Ser536) antibody ICC/IF image

    Immunofluorescence: methanol-fixed MEF cells stained with ARG51518 anti-NFkB p65 phospho (Ser536) antibody.

Customer's Feedback

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Review for anti-NFkB p65 phospho (Ser536) antibody

Application:WB

Sample:HeLa cells untreated or treated with TNF-alpha at 10, 20 or 30 min

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Intravital microscopic thermometry of rat mammary epithelium by fluorescent nanodiamond

IHC-P / Rat

Takahiro Hamoya et al.
Nanoscale Horizons,  (2024)

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Shionone relieves oxygen-glucose deprivation/reoxygenation induced SH-SY5Y cells injury by inhibiting the p38 MAPK/NF-κB pathway

WB / Human

Xiaoli Zhou et al.
J Cardiothorac Surg,  (2024)

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Echinacoside exerts neuroprotection via suppressing microglial α-synuclein/TLR2/NF-κB/NLRP3 axis in parkinsonian models

WB / Mouse

Xue-Ping Yang et al.
Phytomedicine.,  (2024)

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Aucubin Alleviates Intervertebral Disc Degeneration by Repressing NF-κB-NLRP3 Inflammasome Activation in Endplate Chondrocytes

IHC-P / Mouse

Kaiao Zou et al.
J Inflamm Res.,  (2023)

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TNFAIP3 interacting protein 2 relieves lipopolysaccharide (LPS)-induced inflammatory injury in endometritis by inhibiting NF-kappaB activation

WB / Human

Xinxin Qian et al.
Immun Inflamm Dis.,  (2023)

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Glucocorticoid-induced activation of NOX/ROS/NF-κB signaling in MSCs contributes to the development of GONFH

IHC-P / Rat

Huihui Xu et al.
Apoptosis.,  (2023)

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Downregulation of lncRNA NEAT1 alleviates sepsis-induced acute kidney injury

WB / Human

Yuhua Zhou et al.
Cent Eur J Immunol .,  (2022)

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Elucidation of the Underlying Mechanism of Gujian Oral Liquid Acting on Osteoarthritis through Network Pharmacology, Molecular Docking, and Experiment

IHC-P / Mouse

Congzi Wu et al.
Biomed Res Int.,  (2022)

publication_link

 

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N-Butanol Extract of Modified You-Gui-Yin Attenuates Osteoclastogenesis and Ameliorates Osteoporosis by Inhibiting RANKL-Mediated NF-κB Signaling

IHC-P / Mouse

Qinghe Zeng et al.
Front Endocrinol (Lausanne).,  (2022)

publication_link

 

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Neohesperidin Protects Angiotensin II-Induced Hypertension and Vascular Remodeling.

WB / Mouse

Zhang J et al.
Frontiers in Pharmacology,  (2022)

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Chlorogenic Acid retards cartilaginous endplate degeneration and ameliorates intervertebral disc degeneration via suppressing NF-κB signaling.

IHC-P / Mouse

Qinwen Ge et al.
Life Sci.,  (2021)

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