ARG45205
anti-NDUFB5 antibody
anti-NDUFB5 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NDUFB5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDUFB5 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human NDUFB5. |
| Conjugation | Un-conjugated |
| Alternate Names | NDUFB5; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa; CI-SGDH; SGDH; NADH Dehydrogenase [Ubiquinone] 1 Beta Subcomplex Subunit 5, Mitochondrial; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 5, 16kDa; NADH-Ubiquinone Oxidoreductase SGDH Subunit; Complex I SGDH Subunit; Complex I-SGDH; MGC12314; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 5 (16kD, SGDH); CISGDH |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 22 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.01% Sodium azide and 4% Trehalose. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O43674 Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial Swiss-port # Q9CQH3 Mouse NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial |
|---|---|
| Gene Symbol | NDUFB5 |
| Gene Full Name | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa |
| Background | The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011] |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt] |
| Cellular Localization | Mitochondrion inner membrane. [UniProt] |
| Calculated MW | 22 kDa |
Images (8) Click the Picture to Zoom In
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ARG45205 anti-NDUFB5 antibody IHC-P image
Immunohistochemistry: Human prostate cancer stained with ARG45205 anti-NDUFB5 antibody at 2 μg/ml dilution.
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ARG45205 anti-NDUFB5 antibody ICC/IF image
Immunofluorescence: MCF-7 stained with ARG45205 anti-NDUFB5 antibody at 5 ug/ml dilution.
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ARG45205 anti-NDUFB5 antibody WB image
Western blot: MCF-7, THP-1, and A431 stained with ARG45205 anti-NDUFB5 antibody at 0.5 μg/ml dilution.
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ARG45205 anti-NDUFB5 antibody FACS image
Flow Cytometry: A431 stained with ARG45205 anti-NDUFB5 antibody at 1 µg/10^6 cells dilution.
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ARG45205 anti-NDUFB5 antibody IHC-P image
Immunohistochemistry: Rat cardiac muscle stained with ARG45205 anti-NDUFB5 antibody at 2 μg/ml dilution.
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ARG45205 anti-NDUFB5 antibody WB image
Western blot: Rat heart, rat kidney,and rat liver stained with ARG45205 anti-NDUFB5 antibody at 0.5 μg/ml dilution.
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ARG45205 anti-NDUFB5 antibody IHC-P image
Immunohistochemistry: Mouse cardiac muscle stained with ARG45205 anti-NDUFB5 antibody at 2 μg/ml dilution.
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ARG45205 anti-NDUFB5 antibody WB image
Western blot: Mouse heart, mouse kidney, and mouse liver stained with ARG45205 anti-NDUFB5 antibody at 0.5 μg/ml dilution.
