ARG56959
anti-NANOG antibody [5A10]
anti-NANOG antibody [5A10] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [5A10] recognizes NANOG |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5A10 |
| Isotype | IgG2a, kappa |
| Target Name | NANOG |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-154 of Human Nanog. |
| Conjugation | Un-conjugated |
| Alternate Names | Homeobox transcription factor Nanog; Homeobox protein NANOG; hNanog |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NANOG |
| Gene Full Name | Nanog homeobox |
| Background | The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| Function | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. [UniProt] |
| Calculated MW | 35 kDa |
Images (3) Click the Picture to Zoom In
-
ARG56959 anti-NANOG antibody [5A10] FACS image
Flow Cytometry: Hep3B cell line stained with ARG56959 anti-NANOG antibody [5A10] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.
-
ARG56959 anti-NANOG antibody [5A10] IHC-P image
Immunohistochemistry: Paraffin embedded sections of Human seminoma tissue stained with ARG56959 anti-NANOG antibody [5A10] at 1:50 for 2 hours at RT. Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min.
-
ARG56959 anti-NANOG antibody [5A10] WB image
Western blot: 35 µg of NIH3T3 cell lysate stained with ARG56959 anti-NANOG antibody [5A10] at 1:500.
Specific References
Stem cell characteristics promote aggressiveness of diffuse large B-cell lymphoma.
WB / Human
