ARG62341
anti-Myc tag antibody [Myc.A7]
anti-Myc tag antibody [Myc.A7] for Dot blot,ELISA,ICC/IF,Immunoprecipitation,Western blot and Other
Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
Publication2
Overview
| Product Description | Mouse Monoclonal antibody [Myc.A7] recognizes Myc tag |
|---|---|
| Tested Reactivity | Other |
| Tested Application | Dot, ELISA, ICC/IF, IP, WB |
| Specificity | Recognizes the N-terminal or C-terminal Myc-tagged fusion proteins |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | Myc.A7 |
| Isotype | IgG1 |
| Target Name | Myc tag |
| Immunogen | EQKLISEEDL (Myc) synthetic peptide conjugated to KLH. |
| Conjugation | Un-conjugated |
| Alternate Names | c-Myc; MRTL; MYCC; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; bHLHe39; Myc proto-oncogene protein; Transcription factor p64 |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A Purified |
| Purification Note | Protein A affinity chromatography from mouse ascites fluid. |
| Buffer | 10mM PBS (pH 7.2) and 0.05% Sodium azide |
| Preservative | 0.05% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | MYC |
|---|---|
| Gene Full Name | v-myc avian myelocytomatosis viral oncogene homolog |
| Background | The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008] |
| Function | Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt] |
| Research Area | Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody |
| PTM | Phosphorylated by PRKDC. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome. Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity). |
Images (1) Click the Picture to Zoom In
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ARG62341 anti-Myc tag antibody [Myc.A7] WB image
Western Blot: HEK293 cells transfected with Myc-tagged protein vector; (1) untransfected and (2) transfected stained with ARG62341 anti-Myc tag antibody [Myc.A7] at 1:1000 (1 μg/mL) dilution
Specific References
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