ARG51785
anti-Myc phospho (Ser62) antibody
anti-Myc phospho (Ser62) antibody for ICC/IF,Western blot and Human,Mouse,Rat
Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Myc phospho (Ser62) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Myc |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic phosphopeptide around Ser62 (P-L-S(p)-P-S) of Human Myc. |
| Conjugation | Un-conjugated |
| Alternate Names | c-Myc; MRTL; MYCC; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; bHLHe39; Myc proto-oncogene protein; Transcription factor p64 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with phospho-specific peptide and the non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MYC |
| Gene Full Name | v-myc avian myelocytomatosis viral oncogene homolog |
| Background | Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. |
| Function | Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30012 Phospho Myc Antibody Duo (pS62, pS58) ARG30125 Phospho c-Myc Antibody Duo (Total, pS62) ARG30126 Phospho c-Myc Antibody Panel (Total, pT58, pS62) Related products: Myc antibodies; Myc ELISA Kits; Myc Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 49 kDa |
| PTM | Phosphorylated by PRKDC. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome. Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity). |
Images (2) Click the Picture to Zoom In
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ARG51785 anti-Myc phospho (Ser62) antibody WB image
Western blot: 30 µg of HeLa cell lysates untreated or treated with calyculin A (50nM, 30mins). The blots were stained with ARG51785 anti-Myc phospho (Ser62) antibody at 1:500 dilution.
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ARG51785 anti-Myc phospho (Ser62) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51785 anti-Myc phospho (Ser62) antibody.
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Review for anti-Myc phospho (Ser62) antibody
Application：WB
Sample：HeLa untreated or treated with calyculin A (50nM, 30mins)
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
