ARG67041
anti-MyD88 L265P antibody [SQab30311]
anti-MyD88 L265P antibody [SQab30311] for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Recombinant rabbit Monoclonal antibody [SQab30311] recognizes MyD88 L265P |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Specificity | This antibody is specifically for detects MyD88 L265P mutation |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab30311 |
| Isotype | IgG |
| Target Name | MyD88 L265P |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human MyD88 (L265P). |
| Conjugation | Un-conjugated |
| Protein Full Name | Myeloid differentiation primary response protein MyD88 |
| Alternate Names | MYD88, MYD88 Innate Immune Signal Transduction Adaptor, Myeloid Differentiation Primary Response Protein MyD88, Myeloid Differentiation Primary Response Gene (88), Myeloid Differentiation Primary Response 88, TLR Adaptor MYD88, Mutant Myeloid Differentiation Primary Response 88, MYD88D, IMD68 |
Application Instructions
| Application Suggestion |
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| Application Note | Antigen Retrieval: High temperature and normal pressure mediated was performed using Tris/EDTA buffer pH 9.0 for 15 min. Primary Incubation: Room Temperature for 30 min *The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | HEK293 (infected with MYD88 L265P ) |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05%BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q99836 Human Myeloid differentiation primary response protein MyD88 |
|---|---|
| Gene Symbol | MyD88 |
| Gene Full Name | MYD88 Innate Immune Signal Transduction Adaptor |
| Background | This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010] |
| Function | Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation. [Uniprot] |
| Calculated MW | 33 kDa |
| PTM | Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination. OTUD4 specifically hydrolyzes 'Lys-63'-linked polyubiquitinated MYD88. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG67041 anti-MyD88 L265P antibody [SQab30311] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded HEK293 with L265P mutation (left) and no mutation (right) stained with ARG67041 anti-MyD88 L265P antibody [SQab30311] at 1:200 dilution.
