ARG40447

anti-MYH1 antibody

anti-MYH1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes MYH1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Specificity The antibody might also react to MYH4 (95%), MYH2 (90%) and MTH8 (85%) based on sequence homology analysis.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MYH1
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-100 of Human MYH1 (NP_005954.3).
Conjugation Un-conjugated
Alternate Names Myosin heavy chain IIx/d; MYHa; HEL71; MyHC-IIx/d; Myosin heavy chain 1; MyHC-2x; MyHC-2X/D; Myosin heavy chain, skeletal muscle, adult 1; Myosin heavy chain 2x; Myosin-1; MYHSA1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse heart
Observed Size 260 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17879 Mouse MYH1

GeneID: 4619 Human MYH1

Swiss-port # P12882 Human Myosin-1

Swiss-port # Q5SX40 Mouse Myosin-1

Gene Symbol MYH1
Gene Full Name myosin, heavy chain 1, skeletal muscle, adult
Background Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008]
Function Muscle contraction. [UniProt]
Cellular Localization Cytoplasm, myofibril. Note=Thick filaments of the myofibrils. [UniProt]
Calculated MW 223 kDa

Images (3) Click the Picture to Zoom In

  • ARG40447 anti-MYH1 antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG40447 anti-MYH1 antibody.

  • ARG40447 anti-MYH1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG40447 anti-MYH1 antibody at 1:100 dilution.

  • ARG40447 anti-MYH1 antibody WB image

    Western blot: 25 µg of Mouse heart lysate stained with ARG40447 anti-MYH1 antibody at 1:1000 dilution.