ARG66202
anti-MUC16 / CA125 antibody [SQab1724]
anti-MUC16 / CA125 antibody [SQab1724] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1724] recognizes MUC16 / CA125 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1724 |
| Target Name | MUC16 / CA125 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around aa. 12200‐12300 of MUC16 / CA125. |
| Conjugation | Un-conjugated |
| Alternate Names | Mucin-16; MUC-16; Ovarian carcinoma antigen CA125; CA125; Ovarian cancer-related tumor marker CA125; CA-125 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MUC16 |
| Gene Full Name | mucin 16, cell surface associated |
| Function | Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. [UniProt] |
| Highlight | Related products: MUC16 antibodies; MUC16 ELISA Kits; Related news: Cancer Pathology Markers (SQ clones) |
| Calculated MW | 1519 kDa |
| PTM | Heavily O-glycosylated; expresses both type 1 and type 2 core glycans. Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans. May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space. May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. |
Images (4) Click the Picture to Zoom In
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ARG66202 anti-MUC16 / CA125 antibody [SQab1724] WB image
Western blot: 30 µg of HeLa and A431 cell lysates stained with ARG66202 anti-MUC16 / CA125 antibody [SQab1724] at 1:500 dilution.
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ARG66202 anti-MUC16 / CA125 antibody [SQab1724] FACS image
Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66202 anti-MUC16 / CA125 antibody [SQab1724] (red) at 1:800 dilution in 1x PBS/1% BSA for 30 min at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66202 anti-MUC16 / CA125 antibody [SQab1724] IHC-P image
Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human ovarian cancer tissue stained with ARG66202 anti-MUC16 / CA125 antibody [SQab1724] at 1:400 dilution.
Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
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ARG66202 anti-MUC16 / CA125 antibody [SQab1724] IHC-P image
Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human ovarian cancer tissue stained with ARG66202 anti-MUC16 / CA125 antibody [SQab1724] at 1:400 dilution.
Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
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Review for anti-MUC16 / CA125 antibody [SQab1724]
Application：WB
Sample：HeLa and A431
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
