ARG59928
anti-MRPL28 antibody
anti-MRPL28 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MRPL28 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MRPL28 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-256 of Human MRPL28 (NP_006419.2). |
| Conjugation | Un-conjugated |
| Alternate Names | Melanoma antigen p15; Melanoma-associated antigen recognized by T-lymphocytes; MAAT1; p15; L28mt; MRP-L28; 39S ribosomal protein L28, mitochondrial |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | 293T | ||||||||||
| Observed Size | ~ 31 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13084 Human 39S ribosomal protein L28, mitochondrial |
|---|---|
| Gene Symbol | MRPL28 |
| Gene Full Name | mitochondrial ribosomal protein L28 |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008] |
| Cellular Localization | Mitochondrion. [UniProt] |
| Calculated MW | 30 kDa |
Images (5) Click the Picture to Zoom In
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ARG59928 anti-MRPL28 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG59928 anti-MRPL28 antibody.
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ARG59928 anti-MRPL28 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer stained with ARG59928 anti-MRPL28 antibody at 1:100 dilution.
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ARG59928 anti-MRPL28 antibody WB image
Western blot: 25 µg of 293T cell lysate stained with ARG59928 anti-MRPL28 antibody at 1:1000 dilution.
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ARG59928 anti-MRPL28 antibody IP image
Immunoprecipitation: 200 µg extracts of 293T cells immunoprecipitated and stained with ARG59928 anti-MRPL28 antibody at 1:1000 dilution.
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ARG59928 anti-MRPL28 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric cancer stained with ARG59928 anti-MRPL28 antibody at 1:100 dilution.
