ARG54980
anti-MMP9 antibody
anti-MMP9 antibody for Western blot and Human,Mouse
Brain Injury IHC Study antibody
Publication9
Overview
| Product Description | Mouse Monoclonal antibody recognizes MMP9 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 331CT17.4.4 |
| Isotype | IgM |
| Target Name | MMP9 |
| Immunogen | Purified His-tagged MMP9 protein fragment. |
| Conjugation | Un-conjugated |
| Alternate Names | Matrix metalloproteinase-9; 92 kDa gelatinase; MMP-9; Gelatinase B; GELB; CLG4B; MANDP2; EC 3.4.24.35; 92 kDa type IV collagenase |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | Crude Ascites and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MMP9 |
| Gene Full Name | matrix metallopeptidase 9 |
| Background | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008] |
| Function | May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix |
| Highlight | Related products: MMP9 antibodies; MMP9 ELISA Kits; MMP9 Duos / Panels; Anti-Mouse IgM secondary antibodies; Related news: Rat MMP9 ELISA Kit is available in arigo |
| Research Area | Brain Injury IHC Study antibody |
| Calculated MW | 78 kDa |
| PTM | Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated. |
Images (2) Click the Picture to Zoom In
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ARG54980 anti-MMP9 antibody WB image
Western blot: 30 µg of MDAMB231 and MCF7 (negative control) cell lysates stained with ARG54980 anti-MMP9 antibody at 1:500 dilution.
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ARG54980 anti-MMP9 antibody WB image
Western blot: Gastric cancer cells stained with ARG54980 anti-MMP9 antibody.
From Limin Zhang et al. Heliyon (2024), doi: 10.1016/j.heliyon.2024.e30803, Fig. 5. C.
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Review for anti-MMP9 antibody
Application：WB
Sample：MEF
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
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