ARG20154
anti-MMP13 antibody
anti-MMP13 antibody for IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Bovine,Horse,Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MMP13 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Hrs |
| Tested Application | IHC-Fr, IHC-P, IP, WB |
| Specificity | The antibody recognizes the proenzyme (~60 kDa) and the cleaved fragments (34 kDa and 48 kDa) of MMP-13. Reactivity to other species has not been tested. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MMP13 |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide surrounding amino acid 454 of rat MMP-13 |
| Conjugation | Un-conjugated |
| Alternate Names | MMP13; Matrix Metallopeptidase 13; Collagenase 3; CLG3; Matrix Metalloproteinase 13 (Collagenase 3); MMP-13; Matrix Metalloproteinase-13; EC 3.4.24.35; EC 3.4.24.22; EC 3.4.24.24; EC 3.4.24.65; EC 3.4.24.; EC 3.4.24.7; EC 3.4.24; MANDP1; MDST |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified Antibody |
| Buffer | PBS, 30% Glycerol, 0.5% BSA and 0.01% Thimerosal |
| Preservative | 0.01% Thimerosal |
| Stabilizer | 30% Glycerol, 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MMP13 |
| Gene Full Name | matrix metallopeptidase 13 |
| Background | This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016] |
| Function | Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion. [UniProt] |
| Cellular Localization | Extracellular matrix, Secreted. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| Calculated MW | 54 kDa |
| PTM | Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG20154 anti-MMP13 antibody WB image
Western blot: Jurkat cell lysate stained with ARG20154 anti-MMP13 antibody.
The antibody recognizes the proenzyme (~60 kDa) and the cleaved fragments (34 kDa and 48 kDa) of MMP-13.
Customer's Feedback
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Review for anti-MMP13 antibody
Application：WB
Sample：A: MDA-MB-231, B: MCF-7
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
