Overview

Product Description	Rabbit Polyclonal antibody recognizes MLKL
Tested Reactivity	Hu, Ms
Tested Application	ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	MLKL
Antigen Species	Human
Immunogen	Recombinant protein of Human MLKL.
Conjugation	Un-conjugated
Alternate Names	Mixed lineage kinase domain-like protein; hMLKL

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HeLa

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 197259 Human MLKL GeneID: 74568 Mouse MLKL Swiss-port # Q8NB16 Human Mixed lineage kinase domain-like protein Swiss-port # Q9D2Y4 Mouse Mixed lineage kinase domain-like protein
Gene Symbol	MLKL
Gene Full Name	mixed lineage kinase domain-like
Background	This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]
Function	Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. [UniProt]
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Calculated MW	54 kDa
PTM	Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.

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