ARG21065
anti-MHC Class II I E kappa antibody [14-4-4S]
anti-MHC Class II I E kappa antibody [14-4-4S] for Blocking,Cellular activation ,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [14-4-4S] recognizes MHC Class II I E kappa |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | BL, Cell-Act , FACS, ICC/IF, IHC-Fr, IP |
| Specificity | Mouse I-Ek/Rat RT1D. The clone 14-4-4S reacts with the I-Ek class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen. The antibody has been reported to cross-react with the rat MHC class II alloantigen RT1D. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 14-4-4S |
| Isotype | IgG2a, kappa |
| Target Name | MHC Class II I E kappa |
| Antigen Species | Mouse |
| Immunogen | C3H mouse skin graft and splenocytes |
| Conjugation | Un-conjugated |
| Alternate Names | AI323765; H-2Ea; MHC-H2-Ea; H2-Ea; I-Ealpha; H-2 class II histocompatibility antigen, E-U alpha chain; Ia3; E-alpha-f; Ia-3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | BBS (pH 8.2) |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
GeneID: 100504404 Mouse H2-Ea-ps Swiss-port # P14439 Mouse H-2 class II histocompatibility antigen, E-U alpha chain |
|---|---|
| Gene Symbol | H2-Ea-ps |
| Gene Full Name | histocompatibility 2, class II antigen E alpha, pseudogene |
| Background | This locus belongs to the class II major histocompatibility complex (MHC) family of genes, which encode immune response (Ia) antigens that function in the T-cell-dependent immune response. This family member has multiple haplotypes, some of which result in the production of an E-alpha subunit that combines with an E-beta subunit to form a functional E complex at the cell surface. Other haplotypes, including that of the reference genome allele, contain mutations and they thus represent polymorphic pseudogenes that do not produce functional products. These mutations include frameshifting indels, nonsense mutations, and deletions of larger regions. The reference genome haplotype contains a deletion at the 5' end of the gene, including the core promoter region and the transcription start site, and therefore no transcripts result from this haplotype. [provided by RefSeq, Aug 2011] |
| Calculated MW | 29 kDa |
