ARG21071
anti-MHC Class I H2 Db antibody [28-14-8]
anti-MHC Class I H2 Db antibody [28-14-8] for Blocking,Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Mouse
Overview
| Product Description | Mouse Monoclonal antibody [28-14-8] recognizes MHC Class I H2 Db |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | BL, FACS, IHC-Fr, IP |
| Specificity | The clone 28-14-8 binds to the α3 domain of H-2Db in the presence or absence of β2 microglobulin. It cross reacts with the α3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. 28-14-8 can block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 28-14-8 |
| Isotype | IgG2a, kappa |
| Target Name | MHC Class I H2 Db |
| Antigen Species | Mouse |
| Immunogen | C3H.SW mouse splenocytes |
| Conjugation | Un-conjugated |
| Alternate Names | H-2 class I histocompatibility antigen, D-B alpha chain; H2-D; B; H-2D |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | BBS (pH 8.2) |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P01899 Mouse H-2 class I histocompatibility antigen, D-B alpha chain |
|---|---|
| Gene Symbol | H2-D1 |
| Gene Full Name | histocompatibility 2, D region locus 1 |
| Function | Involved in the presentation of foreign antigens to the immune system. [UniProt] |
| Calculated MW | 41 kDa |
Images (1) Click the Picture to Zoom In
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ARG21071 anti-MHC Class I H2 Db antibody [28-14-8] FACS image
Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21071 anti-MHC Class I H2 Db antibody [28-14-8] followed by ARG23802 Goat anti-Mouse IgG2a antibody (FITC) (pre-adsorbed).
