ARG10721
anti-MBNL1 antibody [1H1]
anti-MBNL1 antibody [1H1] for ICC/IF,IHC-Frozen sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [1H1] recognizes MBNL1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Chk |
| Species Does Not React With | Ms, Rat |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1H1 |
| Isotype | IgG1 |
| Target Name | MBNL1 |
| Antigen Species | Human |
| Immunogen | Full-length recombinant Human MBNL1. |
| Conjugation | Un-conjugated |
| Alternate Names | Muscleblind-like protein 1; Triplet-expansion RNA-binding protein; MBNL; EXP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification. |
| Buffer | PBS and 50% Glycerol. |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MBNL1 |
| Gene Full Name | muscleblind-like splicing regulator 1 |
| Background | This gene encodes a member of the muscleblind protein family which was initially described in Drosophila melanogaster. The encoded protein is a C3H-type zinc finger protein that modulates alternative splicing of pre-mRNAs. Muscleblind proteins bind specifically to expanded dsCUG RNA but not to normal size CUG repeats and may thereby play a role in the pathophysiology of myotonic dystrophy. Mice lacking this gene exhibited muscle abnormalities and cataracts. Several alternatively spliced transcript variants have been described but the full-length natures of only some have been determined. The different isoforms are thought to have different binding specificities and/or splicing activities. [provided by RefSeq, Sep 2015] |
| Function | Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues. [UniProt] |
| Calculated MW | 42 kDa |
Images (4) Click the Picture to Zoom In
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ARG10721 anti-MBNL1 antibody [1H1] ICC/IF image
Immunocytochemistry: HeLa cells were stained with ARG10721 anti-MBNL1 antibody [1H1] at 1: 1000. Muscleblind-like protein 1 is present in the nucleus of HeLa cells (red). Co-staining is with a rabbit polyclonal antibody against vimentin (red) reveals cytoplasmic intermediate filaments. Blue is a DNA stain which largely colocalizes with the MBNL1 protein in the nucleus.
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ARG10721 anti-MBNL1 antibody [1H1] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG10721 anti-MBNL1 antibody [1H1] (green) at 1:1000 dilution and costained with ARG10757 anti-Hsp 60 antibody (red) at 1:5000 dilution.
The MBNL1 antibody specifically labels nuclei, while the Hsp 60 antibody reveals protein expressed in mitochondria.
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ARG10721 anti-MBNL1 antibody [1H1] WB image
Western blot: HL60 cell lysate was stained with ARG10721 anti-MBNL1 antibody [1H1] at 1:1000 dilution.
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ARG10721 anti-MBNL1 antibody [1H1] WB image
Western blot: HEK293 (cytosol), HEK293 (nuclear), NIH/3T3 (cytosol), NIH/3T3 (nuclear), HeLa (cytosol) and HeLa (nuclear). The blots were stained with ARG10721 anti-MBNL1 antibody [1H1] (green) at 1:1000 dilution.
MBNL1 protein is predominantly detected in the nuclear enriched fractions. This antibody binds to the Human protein and does not bind the rodent homologue.
